TY - JOUR
T1 - IgG and IgM autoantibody differences in discoid and systemic lupus patients
AU - Chong, Benjamin F.
AU - Tseng, Lin Chiang
AU - Lee, Thomas
AU - Vasquez, Rebecca
AU - Li, Quan Z.
AU - Zhang, Song
AU - Karp, David R.
AU - Olsen, Nancy J.
AU - Mohan, Chandra
N1 - Funding Information:
We thank Jack Cohen, Melissa Costner, Azza Mutwally, and Valerie Branch for aiding in the recruitment of subjects. We are grateful for the technical assistance of Sarah Husseini, Loderick Matthews, and Andrew Pazandak. We thank John Nelson, Alice Chang, and Nicol Bush for their helpful comments on the manuscript. This project was funded in part by NIH CTSA Grant UL1 RR024982 and the American College of Rheumatology/Arthritis Foundation Bridge Funding Grant (BFC).
PY - 2012/12
Y1 - 2012/12
N2 - Systemic lupus erythematosus (SLE) patients with discoid lupus erythematosus (DLE) were reported to have milder disease. To test this observation, we used sandwich arrays containing 98 autoantigens to compare autoantibody profiles of SLE subjects without DLE (DLE-SLE\+) (N = 9), SLE subjects with DLE (DLE\+SLE+) (N = 10), DLE subjects without SLE (DLE+SLE-) (N = 11), and healthy controls (N = 11). We validated differentially expressed autoantibodies using immunoassays in DLE-SLE+ (N = 18), DLE+SLE+ (N = 17), DLE+SLE-(N = 23), and healthy subjects (N = 22). Arrays showed 15 IgG autoantibodies (10 against nuclear antigens) and 4 IgM autoantibodies that were differentially expressed (q-value < 0.05). DLE-SLE+ subjects had higher IgG autoantibodies against double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), doublestranded RNA (dsRNA), histone H2A and H2B, and SS-A (52 kDa) compared with all other groups including DLE+SLE+ subjects (P<0.05). Immunoassays measuring anti-dsDNA,-ssDNA, and-SS-A (52 kDa) IgG autoantibodies showed similar trends (P<0.05). Healthy and DLE+SLE-subjects expressed higher IgM autoantibodies against alpha beta crystallin, lipopolysaccharide, heat-shock cognate 70, and desmoglein-3 compared with DLE+SLE+ and DLE-SLE+ subjects. IgG:IgM ratios of autoantibodies against nuclear antigens progressively rose from healthy to DLE-SLE+ subjects. In conclusion, lower IgG autoantibodies against nuclear antigens in DLE+SLE+ versus DLE-SLE+ subjects suggest that DLE indicates lower disease severity. Higher IgM autoantibodies against selected antigens in healthy and DLE+SLE-subjects may be nonpathogenic.
AB - Systemic lupus erythematosus (SLE) patients with discoid lupus erythematosus (DLE) were reported to have milder disease. To test this observation, we used sandwich arrays containing 98 autoantigens to compare autoantibody profiles of SLE subjects without DLE (DLE-SLE\+) (N = 9), SLE subjects with DLE (DLE\+SLE+) (N = 10), DLE subjects without SLE (DLE+SLE-) (N = 11), and healthy controls (N = 11). We validated differentially expressed autoantibodies using immunoassays in DLE-SLE+ (N = 18), DLE+SLE+ (N = 17), DLE+SLE-(N = 23), and healthy subjects (N = 22). Arrays showed 15 IgG autoantibodies (10 against nuclear antigens) and 4 IgM autoantibodies that were differentially expressed (q-value < 0.05). DLE-SLE+ subjects had higher IgG autoantibodies against double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), doublestranded RNA (dsRNA), histone H2A and H2B, and SS-A (52 kDa) compared with all other groups including DLE+SLE+ subjects (P<0.05). Immunoassays measuring anti-dsDNA,-ssDNA, and-SS-A (52 kDa) IgG autoantibodies showed similar trends (P<0.05). Healthy and DLE+SLE-subjects expressed higher IgM autoantibodies against alpha beta crystallin, lipopolysaccharide, heat-shock cognate 70, and desmoglein-3 compared with DLE+SLE+ and DLE-SLE+ subjects. IgG:IgM ratios of autoantibodies against nuclear antigens progressively rose from healthy to DLE-SLE+ subjects. In conclusion, lower IgG autoantibodies against nuclear antigens in DLE+SLE+ versus DLE-SLE+ subjects suggest that DLE indicates lower disease severity. Higher IgM autoantibodies against selected antigens in healthy and DLE+SLE-subjects may be nonpathogenic.
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U2 - 10.1038/jid.2012.207
DO - 10.1038/jid.2012.207
M3 - Article
C2 - 22763789
AN - SCOPUS:84870514032
SN - 0022-202X
VL - 132
SP - 2770
EP - 2779
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 12
ER -