Abstract
The rapid expansion of molecular screening libraries in size and complexity in the last decade has outpaced the discovery rate of cost-effective strategies to single out reagents with sought-after cellular activities. In addition to representing high-priority therapeutic targets, intensely studied cell signaling systems encapsulate robust reference points for mapping novel chemical activities given our deep understanding of the molecular mechanisms that support their activity. In this chapter, we describe strategies for using transcriptional reporters of several well-interrogated signal transduction pathways coupled with high-throughput biochemical assays to fingerprint novel compounds for drug target identification agendas.
Original language | English (US) |
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Pages (from-to) | 3-16 |
Number of pages | 14 |
Journal | Methods in Molecular Biology |
Volume | 1263 |
DOIs | |
State | Published - 2015 |
Keywords
- Dot blotting
- Kras
- Luciferase assay
- RNAi
- Small-molecule screening
- TP53
- Wnt
ASJC Scopus subject areas
- Molecular Biology
- Genetics