TY - JOUR
T1 - Hypertonic HBOC-201 decreases neutrophil activation after hemorrhagic shock
AU - Rivera-Chávez, Fernando A.
AU - Lu, Amanda
AU - Liu, Ming Mei
AU - Abdalla, Adil
AU - Minei, Joseph P.
N1 - Funding Information:
This study was partially supported by the Biopure Corporation via the donation of Hemopure (HBOC 201). Dr. Fernando A. Rivera-Chávez was supported by NIH NIGMS Grant K08 GM071646.
PY - 2014/2
Y1 - 2014/2
N2 - Objective: To evaluate neutrophil activation after exposure to standard HBC-201 (suspended in lactate Ringer's solution) versus HBOC-201 suspended in hypertonic 7.5% saline solution. Methods: We use plasma and tissue obtained from pigs subjected to controlled hemorrhagic shock and an ex vivo model of stimulated human whole blood. The pigs were resuscitated with the following (n = 8 per group) standard HBOC-201, or hypertonic HBOC-201. We used HTS 7.5%, Ringer's lactate as control resuscitation. Human blood was stimulated with same fluids. We measured the following neutrophil markers; IL-8, H2O 2 in pig plasma, MPO in pig tissue, and H2O2, IL-8, and CD11b/CD18 in human whole blood. Results: H2O2 and IL-8 as well as tissue MPO were significantly decreased in pigs resuscitated with HT-HBOC-201 and HT 7.5%. Ex vivo experiments blood diluted with HTS and HT-HBOC-201 revealed lower expression of CD11b/CD18, H2O2, and IL-8. Blood diluted with HBOC-201 had a higher CD11b/CD18 expression than blood diluted with LR solution. Conclusion: Our in vivo and ex vivo experiments indicate that HBOC-201 suspended in hypertonic 7.5% saline solution is associated with significantly less neutrophil activation when compared to standard HBOC-201 suspended in lactate Ringer's solution.
AB - Objective: To evaluate neutrophil activation after exposure to standard HBC-201 (suspended in lactate Ringer's solution) versus HBOC-201 suspended in hypertonic 7.5% saline solution. Methods: We use plasma and tissue obtained from pigs subjected to controlled hemorrhagic shock and an ex vivo model of stimulated human whole blood. The pigs were resuscitated with the following (n = 8 per group) standard HBOC-201, or hypertonic HBOC-201. We used HTS 7.5%, Ringer's lactate as control resuscitation. Human blood was stimulated with same fluids. We measured the following neutrophil markers; IL-8, H2O 2 in pig plasma, MPO in pig tissue, and H2O2, IL-8, and CD11b/CD18 in human whole blood. Results: H2O2 and IL-8 as well as tissue MPO were significantly decreased in pigs resuscitated with HT-HBOC-201 and HT 7.5%. Ex vivo experiments blood diluted with HTS and HT-HBOC-201 revealed lower expression of CD11b/CD18, H2O2, and IL-8. Blood diluted with HBOC-201 had a higher CD11b/CD18 expression than blood diluted with LR solution. Conclusion: Our in vivo and ex vivo experiments indicate that HBOC-201 suspended in hypertonic 7.5% saline solution is associated with significantly less neutrophil activation when compared to standard HBOC-201 suspended in lactate Ringer's solution.
KW - HBOC-201
KW - Hemoglobin-based oxygen carriers
KW - Hemopure
KW - Hemorrhagic shock
KW - Hypertonic resuscitation
KW - Hypertonic saline 7.5%
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U2 - 10.3109/08941939.2013.826756
DO - 10.3109/08941939.2013.826756
M3 - Article
C2 - 24456255
AN - SCOPUS:84897883135
SN - 0894-1939
VL - 27
SP - 14
EP - 20
JO - Journal of Investigative Surgery
JF - Journal of Investigative Surgery
IS - 1
ER -