Hyperspectral imaging utilizing LCTF and DLP technology for surgical and clinical applications

Karel J. Zuzak, Robert P. Francis, Eleanor F. Wehner, Jack Smith, Maritoni Litorja, David W. Allen, Chad Tracy, Jeffrey A Cadeddu, Edward Livingston

Research output: Chapter in Book/Report/Conference proceedingConference contribution

13 Scopus citations


Two different, already characterized, hyperspectral imaging systems created for visualizing the spatial distribution of tissue oxygenation non-invasively for in vivo clinical use are described. Individual components of both liquid crystal tunable filter (LCTF) and digital light processing (DLP) systems were characterized, calibrated, and found to be well within manufacturer specifications. Coupling LCTF with charge coupled device (CCD) technology and acquiring images at multiple, contiguous wavelengths and at narrow bandwidths are formatted into a hyperspectral data cube consisting of one spectral and two spatial dimensions. DLP® technology has the novel ability to conform light to any desired spectral illumination scheme. Subsequently the collected multispectral data are processed into chemically relevant images that are color encoded at each pixel detector for the relative percentage of oxyhemoglobin. Using spectral illumination methods unique to the DLP hyperspectral imager results in producing chemically relevant images at near video rate; 4 frames per second. As an example, both systems are used to collect spectral data from a 27.22 kg porcine kidney whose renal artery has been occluded for 60 minutes. Both systems return nearly identical spectra collected from the surface of the kidney, with a root mean square deviation between the two spectra of 0.02.

Original languageEnglish (US)
Title of host publicationDesign and Quality for Biomedical Technologies II
ISBN (Print)9780819474162
StatePublished - Jan 1 2009
EventDesign and Quality for Biomedical Technologies II - San Jose, CA, United States
Duration: Jan 26 2009Jan 26 2009

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherDesign and Quality for Biomedical Technologies II
Country/TerritoryUnited States
CitySan Jose, CA


  • Digital light processing (DLP)
  • Digital micromirror device (DMD)
  • Hyperspectral imaging
  • Liquid crystal tunable filter (LCTF)
  • Optical biopsy
  • Renal artery occlusion

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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