TY - JOUR
T1 - Horseradish peroxidase acute ototoxicity and the uptake and movement of the peroxidase in the auditory system of the Guinea pig
AU - Ross, Muriel D.
AU - Nuttall, A. L.
AU - Wright, Ch G.
N1 - Funding Information:
This work was supported by Public Health Service Grants NS 11731 and NS 05785. Present address, Callier Center for Communication Disorders, University of Texas at Dallas, Texas 75235, USA.
PY - 1977
Y1 - 1977
N2 - When guinea pig cochlea was perfused in vivo with a solution of 1 % horseradish peroxidase (HRP) in artificial perilymph, the enzyme was found in the basilar membrane, spiral limbus, some outer and inner hair cells and some supporting cells and it was gradually cleared away with time. Acute signs of cell damage included swelling, vacuolization and diffuse labeling of some hair cells, but stereocilia remained normal in configuration. Albino melanocytes of the spiral ligament were also damaged, and vacuolization of Reissner's membrane occurred after 10% HRP. Both concentrations caused a gradual decline in CM, showing that HRP is acutely oto-toxic but its mode of action is unknown. No retrograde transport of HRP to spiral ganglion cells or to brain stem neurons occurred, but some brain stem neurons took up HRP from the neuropil following diffusion from the cochlea.
AB - When guinea pig cochlea was perfused in vivo with a solution of 1 % horseradish peroxidase (HRP) in artificial perilymph, the enzyme was found in the basilar membrane, spiral limbus, some outer and inner hair cells and some supporting cells and it was gradually cleared away with time. Acute signs of cell damage included swelling, vacuolization and diffuse labeling of some hair cells, but stereocilia remained normal in configuration. Albino melanocytes of the spiral ligament were also damaged, and vacuolization of Reissner's membrane occurred after 10% HRP. Both concentrations caused a gradual decline in CM, showing that HRP is acutely oto-toxic but its mode of action is unknown. No retrograde transport of HRP to spiral ganglion cells or to brain stem neurons occurred, but some brain stem neurons took up HRP from the neuropil following diffusion from the cochlea.
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U2 - 10.3109/00016487709123957
DO - 10.3109/00016487709123957
M3 - Article
C2 - 906813
AN - SCOPUS:0017739961
SN - 0001-6489
VL - 84
SP - 187
EP - 201
JO - Acta Oto-Laryngologica
JF - Acta Oto-Laryngologica
IS - 1-6
ER -