Histopathology of rejected orthotopic corneal grafts in the rat

D. G. Callanan, M. W. Luckenbach, B. J. Fischer, J. S. Peeler, J. Y. Niederkorn

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


We have used an orthotopic graft model in the rat to study the histologic characteristics of corneal allograft rejection. Unrejected allogeneic grafts could not be distinguished from clear syngeneic grafts. Although donor Langerhans cells are necessary for the development of delayed-type hypersensitivity (DTH), the histopathological characteristics of rejecting corneal allografts in immunologically naive hosts were identical regardless of the presence or absence of donor Langerhans cells. By contrast, preimmunization had a dramatic effect on the histology of graft rejection. Untreated allografts placed onto pre-immunized recipients underwent a marked cellular necrosis accompanied by minimal inflammation that easily distinguished these grafts from the previous groups. These results suggest that neither the presence nor absence of DTH responsiveness correlates with the histopathological events that accompany corneal graft rejection. However, preimmunization leads to a different histologiv pattern of rejection that is characterized by an intense cellular necrosis.

Original languageEnglish (US)
Pages (from-to)413-424
Number of pages12
JournalInvestigative Ophthalmology and Visual Science
Issue number3
StatePublished - 1989

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


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