TY - JOUR
T1 - Hexapeptide Tandem Repeats Dictate the Formation of Silkmoth Chorion, a Natural Protective Amyloid
AU - Tsiolaki, Paraskevi L.
AU - Louros, Nikolaos N.
AU - Iconomidou, Vassiliki A.
N1 - Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2018/10/12
Y1 - 2018/10/12
N2 - Silkmoth chorion is a fibrous structure composed mainly of two major protein classes, families A and B. Both families of silkmoth chorion proteins present a highly conserved, in sequence and in length, central domain, consisting of Gly-rich tandem hexapeptide repetitive segments, flanked by two more variable N-terminal and C-terminal arms. Primary studies identified silkmoth chorion as a functional protective amyloid by unveiling the amyloidogenic properties of the central domain of both protein families. In this work, we attempt to detect the principal source of amyloidogenicity of the central domain by focusing on the role of the tandem hexapeptide sequence repeats. Concurrently, we discuss a possible mechanism for the self-assembly of class A protofilaments, suggesting that the aggregation-prone hexapeptide building blocks may fold into a triangle-shaped β-helical structure.
AB - Silkmoth chorion is a fibrous structure composed mainly of two major protein classes, families A and B. Both families of silkmoth chorion proteins present a highly conserved, in sequence and in length, central domain, consisting of Gly-rich tandem hexapeptide repetitive segments, flanked by two more variable N-terminal and C-terminal arms. Primary studies identified silkmoth chorion as a functional protective amyloid by unveiling the amyloidogenic properties of the central domain of both protein families. In this work, we attempt to detect the principal source of amyloidogenicity of the central domain by focusing on the role of the tandem hexapeptide sequence repeats. Concurrently, we discuss a possible mechanism for the self-assembly of class A protofilaments, suggesting that the aggregation-prone hexapeptide building blocks may fold into a triangle-shaped β-helical structure.
KW - functional amyloids
KW - silkmoth chorion proteins
KW - silkmoth eggshell
KW - tandem repeats
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U2 - 10.1016/j.jmb.2018.06.042
DO - 10.1016/j.jmb.2018.06.042
M3 - Article
C2 - 29964045
AN - SCOPUS:85054027082
SN - 0022-2836
VL - 430
SP - 3774
EP - 3783
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 20
ER -