TY - JOUR
T1 - HBV C promoter Sp1 binding sequence functionally substitutes for the yeast ARS1 ABF1 binding site
AU - Yan, Peijun
AU - Mao, Xicheng
AU - Wang, Lei
AU - Zha, Xiliang
AU - Lu, Changde
PY - 2002
Y1 - 2002
N2 - Transcriptional factors have been implicated in eukaryotic DNA replication. We have studied the potential function of a viral promoter sequence in DNA replication. The hepatitis B virus (HBV) pregenomic promoter is regulated by two enhancers and cis-elements. The G-C rich region between 1734-1754 nt, which contains two SP1 binding sites, is necessary for transcription origin and HBV replication. We found that the Abf1-binding B3 element in yeast ARS1 can be functionally replaced by the viral Sp1-binding DNA sequence, which activates transcription from the HBV C promoter. Further, yeast RAP1 bound to the viral Sp1 binding sites in vitro. These results suggest that RAP1 binds to the Sp1 binding sites and stimulates yeast DNA replication.
AB - Transcriptional factors have been implicated in eukaryotic DNA replication. We have studied the potential function of a viral promoter sequence in DNA replication. The hepatitis B virus (HBV) pregenomic promoter is regulated by two enhancers and cis-elements. The G-C rich region between 1734-1754 nt, which contains two SP1 binding sites, is necessary for transcription origin and HBV replication. We found that the Abf1-binding B3 element in yeast ARS1 can be functionally replaced by the viral Sp1-binding DNA sequence, which activates transcription from the HBV C promoter. Further, yeast RAP1 bound to the viral Sp1 binding sites in vitro. These results suggest that RAP1 binds to the Sp1 binding sites and stimulates yeast DNA replication.
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U2 - 10.1089/104454902760599717
DO - 10.1089/104454902760599717
M3 - Article
C2 - 12443543
AN - SCOPUS:0036438720
SN - 1044-5498
VL - 21
SP - 737
EP - 742
JO - DNA and Cell Biology
JF - DNA and Cell Biology
IS - 10
ER -