Abstract
Previously, we demonstrated that SGEF enhances EGFR stability; however, SGEF-mediated downstream signaling of EGFR is not well understood. Here, we show that SGEF enhances EGF-induced ERK1/2 activation independent of its guanine nucleotide exchange (GEF) activity. We further show that SGEF interacts with Grb2, a critical downstream transducer of EGFR. Surprisingly, we found that interaction of Grb2 to SGEF antagonizes the ability of SGEF to enhance EGF-induced ERK1/2 activation. Taken together, this study reports a novel function of SGEF that excludes GEF and also provides important insights into the complex role of Grb2 in EGFR signal transduction.
Original language | English (US) |
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Pages (from-to) | 239-247 |
Number of pages | 9 |
Journal | Molecular and Cellular Biochemistry |
Volume | 389 |
Issue number | 1-2 |
DOIs | |
State | Published - Apr 2014 |
Externally published | Yes |
Keywords
- EGF
- EGFR
- ERK1/2
- Grb2
- SGEF
ASJC Scopus subject areas
- Molecular Biology
- Clinical Biochemistry
- Cell Biology