TY - JOUR
T1 - Going forward with genetics
T2 - Recent technological advances and forward genetics in mice
AU - Moresco, Eva Marie Y
AU - Li, Xiaohong
AU - Beutler, Bruce
N1 - Funding Information:
Supported by NIH grants HHSN272200700038C and AI100627-01 (B.B.).
PY - 2013/5
Y1 - 2013/5
N2 - Forward genetic analysis is an unbiased approach for identifying genes essential to defined biological phenomena. When applied to mice, it is one of the most powerful methods to facilitate understanding of the genetic basis of human biology and disease. The speed at which disease-causing mutations can be identified in mutagenized mice has been markedly increased by recent advances in DNA sequencing technology. Creating and analyzing mutant phenotypes may therefore become rate-limiting in forward genetic experimentation. We review the forward genetic approach and its future in the context of recent technological advances, in particular massively parallel DNA sequencing, induced pluripotent stem cells, and haploid embryonic stem cells.
AB - Forward genetic analysis is an unbiased approach for identifying genes essential to defined biological phenomena. When applied to mice, it is one of the most powerful methods to facilitate understanding of the genetic basis of human biology and disease. The speed at which disease-causing mutations can be identified in mutagenized mice has been markedly increased by recent advances in DNA sequencing technology. Creating and analyzing mutant phenotypes may therefore become rate-limiting in forward genetic experimentation. We review the forward genetic approach and its future in the context of recent technological advances, in particular massively parallel DNA sequencing, induced pluripotent stem cells, and haploid embryonic stem cells.
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U2 - 10.1016/j.ajpath.2013.02.002
DO - 10.1016/j.ajpath.2013.02.002
M3 - Article
C2 - 23608223
AN - SCOPUS:84876549173
SN - 0002-9440
VL - 182
SP - 1462
EP - 1473
JO - American Journal of Pathology
JF - American Journal of Pathology
IS - 5
ER -