TY - JOUR
T1 - Genome-wide functional analyses of plant coiled–coil NLR-type pathogen receptors reveal essential roles of their N-terminal domain in oligomerization, networking, and immunity
AU - Wróblewski, Tadeusz
AU - Spiridon, Laurentiu
AU - Martin, Eliza Cristina
AU - Petrescu, Andrei Jose
AU - Cavanaugh, Keri
AU - Truco, Maria José
AU - Xu, Huaqin
AU - Gozdowski, Dariusz
AU - Pawłowski, Krzysztof
AU - Michelmore, Richard W.
AU - Takken, Frank L.W.
N1 - Publisher Copyright:
© 2018 Wróblewski et al. http://creativecommons.org/licenses/by/4.0/.
PY - 2018/12
Y1 - 2018/12
N2 - The ability to induce a defense response after pathogen attack is a critical feature of the immune system of any organism. Nucleotide-binding leucine-rich repeat receptors (NLRs) are key players in this process and perceive the occurrence of nonself-activities or foreign molecules. In plants, coevolution with a variety of pests and pathogens has resulted in repertoires of several hundred diverse NLRs in single individuals and many more in populations as a whole. However, the mechanism by which defense signaling is triggered by these NLRs in plants is poorly understood. Here, we show that upon pathogen perception, NLRs use their N-terminal domains to transactivate other receptors. Their N-terminal domains homo- and heterodimerize, suggesting that plant NLRs oligomerize upon activation, similar to the vertebrate NLRs; however, consistent with their large number in plants, the complexes are highly heterometric. Also, in contrast to metazoan NLRs, the N-terminus, rather than their centrally located nucleotide-binding (NB) domain, can mediate initial partner selection. The highly redundant network of NLR interactions in plants is proposed to provide resilience to perturbation by pathogens.
AB - The ability to induce a defense response after pathogen attack is a critical feature of the immune system of any organism. Nucleotide-binding leucine-rich repeat receptors (NLRs) are key players in this process and perceive the occurrence of nonself-activities or foreign molecules. In plants, coevolution with a variety of pests and pathogens has resulted in repertoires of several hundred diverse NLRs in single individuals and many more in populations as a whole. However, the mechanism by which defense signaling is triggered by these NLRs in plants is poorly understood. Here, we show that upon pathogen perception, NLRs use their N-terminal domains to transactivate other receptors. Their N-terminal domains homo- and heterodimerize, suggesting that plant NLRs oligomerize upon activation, similar to the vertebrate NLRs; however, consistent with their large number in plants, the complexes are highly heterometric. Also, in contrast to metazoan NLRs, the N-terminus, rather than their centrally located nucleotide-binding (NB) domain, can mediate initial partner selection. The highly redundant network of NLR interactions in plants is proposed to provide resilience to perturbation by pathogens.
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U2 - 10.1371/journal.pbio.2005821
DO - 10.1371/journal.pbio.2005821
M3 - Article
C2 - 30540748
AN - SCOPUS:85059257726
SN - 1544-9173
VL - 16
JO - PLoS biology
JF - PLoS biology
IS - 12
M1 - e2005821
ER -