Generation of a hybrid sequence-specific single-stranded deoxyribonuclease

D. R. Corey, P. G. Schultz

Research output: Contribution to journalArticlepeer-review

135 Scopus citations


The relatively nonspecific single-stranded deoxyribonuclease, staphylococcal nuclease, was selectively fused to an oligonucleotide binding site of defined sequence to generate a hybrid enzyme. A cysteine was substituted for Lys116 in the enzyme by oligonucleotide-directed mutagenesis and coupled to an oligonucleotide that contained a 3′-thiol. The resulting hybrid enzyme cleaved single-stranded DNA at sites adjacent to the oligonucleotide binding site.

Original languageEnglish (US)
Pages (from-to)1401-1403
Number of pages3
Issue number4832
StatePublished - 1987

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Generation of a hybrid sequence-specific single-stranded deoxyribonuclease'. Together they form a unique fingerprint.

Cite this