TY - JOUR
T1 - Gene structure of murine Gna11 and Gna15
T2 - Tandemly duplicated Gq class G protein α subunit genes
AU - Davignon, Isabelle
AU - Barnard, Mary
AU - Gavrilova, Oksana
AU - Sweet, Kimberly
AU - Wilkie, Thomas M.
N1 - Funding Information:
We thank F. Kolakowski and S. Yokoyama for constructing phylogenetic trees, for illuminating discussions, and, in addition to E. Ross, A. Beuve, H. J. Fulle, and our colleagues, for critical reading of this manuscript; L. Brundage, D. Smith, and A. Spiegel for communicating unpublished data, W. Fitch for discussions, and J. Satter-®eld for help with preparation of the manuscript. This work was supported in part by a Pharmacological Sciences Training Grant from the National Institutes of Health (NIH) to I.D. and by grants to T.M.W. from the NIH, the Texas Advanced Research Program, the Leukemia Association of North Central Texas, and a Basil O'Connor Award.
PY - 1996/2/1
Y1 - 1996/2/1
N2 - G protein α subunits are encoded by a multigene family of 16 genes that can be grouped into four classes, Gq, Gs, Gi, and G12. The Gq class is composed of four genes in mouse and human, and two of these genes, Gna11 and Gna15, cosegregate on mouse chromosome 10. We have characterized the gene structures of murine Gna11 and Gna15. The two genes are tandemly duplicated in a head-to-tail array. The upstream gene, Gna11, is ubiquitously expressed, whereas expression of the downstream gene, Gna15, is restricted to hematopoietic cells. The coding sequence of each gene is contained within seven exons, and the two genes together span 43 kb, separated by 6 kb of intergenic region. We have found no evidence for alternative splicing within the coding sequence of either gene. Sequence alignments show that the positions of the six intervening sequences are conserved in the two genes, consistent with Gna11 and Gna15 arising by tandem duplication from a common progenitor gene in vertebrates. Phylogenetic trees reveal unequal evolutionary rates among α subunits of the Gq class. The rate of change is approximately six fold higher in Gna15 than in Gna11.
AB - G protein α subunits are encoded by a multigene family of 16 genes that can be grouped into four classes, Gq, Gs, Gi, and G12. The Gq class is composed of four genes in mouse and human, and two of these genes, Gna11 and Gna15, cosegregate on mouse chromosome 10. We have characterized the gene structures of murine Gna11 and Gna15. The two genes are tandemly duplicated in a head-to-tail array. The upstream gene, Gna11, is ubiquitously expressed, whereas expression of the downstream gene, Gna15, is restricted to hematopoietic cells. The coding sequence of each gene is contained within seven exons, and the two genes together span 43 kb, separated by 6 kb of intergenic region. We have found no evidence for alternative splicing within the coding sequence of either gene. Sequence alignments show that the positions of the six intervening sequences are conserved in the two genes, consistent with Gna11 and Gna15 arising by tandem duplication from a common progenitor gene in vertebrates. Phylogenetic trees reveal unequal evolutionary rates among α subunits of the Gq class. The rate of change is approximately six fold higher in Gna15 than in Gna11.
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U2 - 10.1006/geno.1996.0059
DO - 10.1006/geno.1996.0059
M3 - Article
C2 - 8838318
AN - SCOPUS:0030066936
SN - 0888-7543
VL - 31
SP - 359
EP - 366
JO - Genomics
JF - Genomics
IS - 3
ER -