GEC1, a protein related to GABARAP, interacts with tubulin and GABA A receptor

Virginie Mansuy; Wilfrid Boireau; Annick Fraichard; Jean Luc Schlick; Michèle Jouvenot; Régis Delage-Mourroux

doi:10.1016/j.bbrc.2004.10.072

GEC1, a protein related to GABARAP, interacts with tubulin and GABA _A receptor

Virginie Mansuy, Wilfrid Boireau, Annick Fraichard, Jean Luc Schlick, Michèle Jouvenot, Régis Delage-Mourroux

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

We have previously identified in uterine cells a novel estrogen-regulated gene called gec1. GEC1 presents 87% identity with GABARAP which, so far, was the only protein found to associate with tubulin and GABA _A receptor. We demonstrated then that GEC1 interacts in vitro with tubulin and GABA _A receptor, and promotes tubulin assembly and microtubule bundling. Since all polyclonal antibodies failed in discrimination of both proteins GEC1 and GABARAP, a GEC1-GFP fusion protein was used to specifically localize GEC1. GEC1-GFP was distributed over the cytoplasm in perinuclear vesicles with a scattered pattern. Overall, our data show that GEC1 could be a new member of the GABARAP family involved in the transport of GABA _A receptor.

Original language	English (US)
Pages (from-to)	639-648
Number of pages	10
Journal	Biochemical and Biophysical Research Communications
Volume	325
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2004.10.072
State	Published - Dec 10 2004

Keywords

Biacore
GABARAP
GEC1
GST-pull-down
Protein trafficking
Protein-protein interaction

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2004.10.072

Cite this

@article{099173ddf9954080bb6da2c7f1eb8696,

title = "GEC1, a protein related to GABARAP, interacts with tubulin and GABA A receptor",

abstract = "We have previously identified in uterine cells a novel estrogen-regulated gene called gec1. GEC1 presents 87% identity with GABARAP which, so far, was the only protein found to associate with tubulin and GABA A receptor. We demonstrated then that GEC1 interacts in vitro with tubulin and GABA A receptor, and promotes tubulin assembly and microtubule bundling. Since all polyclonal antibodies failed in discrimination of both proteins GEC1 and GABARAP, a GEC1-GFP fusion protein was used to specifically localize GEC1. GEC1-GFP was distributed over the cytoplasm in perinuclear vesicles with a scattered pattern. Overall, our data show that GEC1 could be a new member of the GABARAP family involved in the transport of GABA A receptor.",

keywords = "Biacore, GABARAP, GEC1, GST-pull-down, Protein trafficking, Protein-protein interaction",

author = "Virginie Mansuy and Wilfrid Boireau and Annick Fraichard and Schlick, {Jean Luc} and Mich{\`e}le Jouvenot and R{\'e}gis Delage-Mourroux",

note = "Funding Information: This research work was supported by a fellowship from the Minist{\`e}re de l{\textquoteright}Enseignement Sup{\'e}rieur et de la Recherche and by grants from the Minist{\`e}re de l{\textquoteright}Enseignement Sup{\'e}rieur et de la Recherche (E.A. 3182) and from the Ligue Nationale Contre le Cancer (Comit{\'e}s du Jura et du Doubs). We are particularly grateful to Dr. M.F. Carlier (Gif sur Yvette, France) for her cooperation and for providing us tubulin purified by phosphocellulose chromatography. We are also grateful to Dr. R. Olsen (Los Angeles, CA, USA) for generous gift of GABARAP plasmids and to Dr. J.M. Fritschy (Zurich, Switzerland) for providing us guinea-pig antiserum against the GABA A receptor γ2 subunit. We thank C. Qu{\'e}tard and D. To Van from Biacore International SA for their help in surface plasmon resonance instrumentation (SPR).",

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T1 - GEC1, a protein related to GABARAP, interacts with tubulin and GABA A receptor

AU - Mansuy, Virginie

AU - Boireau, Wilfrid

AU - Fraichard, Annick

AU - Schlick, Jean Luc

AU - Jouvenot, Michèle

AU - Delage-Mourroux, Régis

N1 - Funding Information: This research work was supported by a fellowship from the Ministère de l’Enseignement Supérieur et de la Recherche and by grants from the Ministère de l’Enseignement Supérieur et de la Recherche (E.A. 3182) and from the Ligue Nationale Contre le Cancer (Comités du Jura et du Doubs). We are particularly grateful to Dr. M.F. Carlier (Gif sur Yvette, France) for her cooperation and for providing us tubulin purified by phosphocellulose chromatography. We are also grateful to Dr. R. Olsen (Los Angeles, CA, USA) for generous gift of GABARAP plasmids and to Dr. J.M. Fritschy (Zurich, Switzerland) for providing us guinea-pig antiserum against the GABA A receptor γ2 subunit. We thank C. Quétard and D. To Van from Biacore International SA for their help in surface plasmon resonance instrumentation (SPR).

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Y1 - 2004/12/10

N2 - We have previously identified in uterine cells a novel estrogen-regulated gene called gec1. GEC1 presents 87% identity with GABARAP which, so far, was the only protein found to associate with tubulin and GABA A receptor. We demonstrated then that GEC1 interacts in vitro with tubulin and GABA A receptor, and promotes tubulin assembly and microtubule bundling. Since all polyclonal antibodies failed in discrimination of both proteins GEC1 and GABARAP, a GEC1-GFP fusion protein was used to specifically localize GEC1. GEC1-GFP was distributed over the cytoplasm in perinuclear vesicles with a scattered pattern. Overall, our data show that GEC1 could be a new member of the GABARAP family involved in the transport of GABA A receptor.

AB - We have previously identified in uterine cells a novel estrogen-regulated gene called gec1. GEC1 presents 87% identity with GABARAP which, so far, was the only protein found to associate with tubulin and GABA A receptor. We demonstrated then that GEC1 interacts in vitro with tubulin and GABA A receptor, and promotes tubulin assembly and microtubule bundling. Since all polyclonal antibodies failed in discrimination of both proteins GEC1 and GABARAP, a GEC1-GFP fusion protein was used to specifically localize GEC1. GEC1-GFP was distributed over the cytoplasm in perinuclear vesicles with a scattered pattern. Overall, our data show that GEC1 could be a new member of the GABARAP family involved in the transport of GABA A receptor.

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