Galactosyltransferase activities in human sera: Detection of a cancer-associated isoenzyme

Daniel K. Podolsky; Milton M. Weiser

doi:10.1016/S0006-291X(75)80181-1

Galactosyltransferase activities in human sera: Detection of a cancer-associated isoenzyme

Daniel K. Podolsky, Milton M. Weiser

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Abstract

Sera from normal subjects and cancer patients were evaluated for galactosyltransferase activity including kinetic and electrophoretic properties. Discontinuous polyacrylamide electrophoresis of whole sera demonstrated the presence of an additional peak of enzyme activity in cancer sera. The majority of recovered enzymatic activity in both normal and cancer sera ran as a broad band of activity behind an albumin standard; the cancerassociated peak was slower moving. Techniques have been developed for the rapid assay of galactosyltransferase activity from gel slices which permit detection of both peaks of activity.

Original language	English (US)
Pages (from-to)	545-551
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	65
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(75)80181-1
State	Published - Jul 22 1975

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Galactosyltransferase activities in human sera: Detection of a cancer-associated isoenzyme",

abstract = "Sera from normal subjects and cancer patients were evaluated for galactosyltransferase activity including kinetic and electrophoretic properties. Discontinuous polyacrylamide electrophoresis of whole sera demonstrated the presence of an additional peak of enzyme activity in cancer sera. The majority of recovered enzymatic activity in both normal and cancer sera ran as a broad band of activity behind an albumin standard; the cancerassociated peak was slower moving. Techniques have been developed for the rapid assay of galactosyltransferase activity from gel slices which permit detection of both peaks of activity.",

author = "Podolsky, {Daniel K.} and Weiser, {Milton M.}",

note = "Funding Information: We thank Dr. Kurt Jo Isselbacher for his support in this work and his critical review of this manuscript. This work was supported by a grant from the American Cancer Society {BC-93), Massachusetts Division American Cancer Society Cancer Scholar Award and National Institute of Health Grant (CA-16703).",

year = "1975",

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T1 - Galactosyltransferase activities in human sera

T2 - Detection of a cancer-associated isoenzyme

AU - Podolsky, Daniel K.

AU - Weiser, Milton M.

N1 - Funding Information: We thank Dr. Kurt Jo Isselbacher for his support in this work and his critical review of this manuscript. This work was supported by a grant from the American Cancer Society {BC-93), Massachusetts Division American Cancer Society Cancer Scholar Award and National Institute of Health Grant (CA-16703).

PY - 1975/7/22

Y1 - 1975/7/22

N2 - Sera from normal subjects and cancer patients were evaluated for galactosyltransferase activity including kinetic and electrophoretic properties. Discontinuous polyacrylamide electrophoresis of whole sera demonstrated the presence of an additional peak of enzyme activity in cancer sera. The majority of recovered enzymatic activity in both normal and cancer sera ran as a broad band of activity behind an albumin standard; the cancerassociated peak was slower moving. Techniques have been developed for the rapid assay of galactosyltransferase activity from gel slices which permit detection of both peaks of activity.

AB - Sera from normal subjects and cancer patients were evaluated for galactosyltransferase activity including kinetic and electrophoretic properties. Discontinuous polyacrylamide electrophoresis of whole sera demonstrated the presence of an additional peak of enzyme activity in cancer sera. The majority of recovered enzymatic activity in both normal and cancer sera ran as a broad band of activity behind an albumin standard; the cancerassociated peak was slower moving. Techniques have been developed for the rapid assay of galactosyltransferase activity from gel slices which permit detection of both peaks of activity.

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Galactosyltransferase activities in human sera: Detection of a cancer-associated isoenzyme

Abstract

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