TY - JOUR
T1 - Forward genetic screens to identify circadian rhythm mutants in mice
AU - Siepka, Sandra M.
AU - Takahashi, Joseph S.
N1 - Funding Information:
This work was supported by NIH grant #U01_MH61915. J. S. Takahashi is an Investigator in the Howard Hughes Medical Institute.
PY - 2005
Y1 - 2005
N2 - This article describes the methods and techniques used to produce mutagenized mice to conduct high-throughput forward genetic screens for circadian rhythm mutants in the mouse. In particular, we outline methods to safely prepare and administer the chemical mutagen N-nitroso-N-ethylurea (ENU) to mice. We also discuss the importance of selecting mouse strain and outline breeding strategies, logistics, and throughput to produce these mutant mice. Finally, we discuss the breeding strategies that we use to confirm mutation heritability.
AB - This article describes the methods and techniques used to produce mutagenized mice to conduct high-throughput forward genetic screens for circadian rhythm mutants in the mouse. In particular, we outline methods to safely prepare and administer the chemical mutagen N-nitroso-N-ethylurea (ENU) to mice. We also discuss the importance of selecting mouse strain and outline breeding strategies, logistics, and throughput to produce these mutant mice. Finally, we discuss the breeding strategies that we use to confirm mutation heritability.
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U2 - 10.1016/S0076-6879(05)93007-3
DO - 10.1016/S0076-6879(05)93007-3
M3 - Article
C2 - 15817290
AN - SCOPUS:16844375557
SN - 0076-6879
VL - 393
SP - 219
EP - 229
JO - Methods in Enzymology
JF - Methods in Enzymology
M1 - 7
ER -