TY - JOUR
T1 - Fibronectin and fibrinolysis are not required for fibrin gel contraction by human skin fibroblasts
AU - Tuan, T. L.
AU - Grinnell, F.
PY - 1989/9
Y1 - 1989/9
N2 - Human skin fibroblasts contracted fibrin gels in a time‐ and cell‐dependent manner. Under optimal conditions, gel contraction amounted to more than 50% in 2 hr. Fibronectin did not promote contraction, and fibrinolysis was not required for contraction, although gels contracted without serum or aprotinin were lysed. Before contraction, fibrin was present in loosely packed, randomly organized fibrils. After contraction, the fibrils were more densely packed and aligned in the plane of cell spreading. Cycloheximide treatment of fibroblasts inhibited gel contraction in serum‐free medium but not in serum‐containing medium. Fibronectin could not substitute for serum in overcoming the cycloheximide effect. Binding sites for fibrin were distributed randomly over the cells' surfaces based on electron microscopic observations. Often small groups of fibrils were localized in indentations at the cell surface. Finally, peptides containing the arg‐gly‐asp‐ser sequence inhibited gel contraction.
AB - Human skin fibroblasts contracted fibrin gels in a time‐ and cell‐dependent manner. Under optimal conditions, gel contraction amounted to more than 50% in 2 hr. Fibronectin did not promote contraction, and fibrinolysis was not required for contraction, although gels contracted without serum or aprotinin were lysed. Before contraction, fibrin was present in loosely packed, randomly organized fibrils. After contraction, the fibrils were more densely packed and aligned in the plane of cell spreading. Cycloheximide treatment of fibroblasts inhibited gel contraction in serum‐free medium but not in serum‐containing medium. Fibronectin could not substitute for serum in overcoming the cycloheximide effect. Binding sites for fibrin were distributed randomly over the cells' surfaces based on electron microscopic observations. Often small groups of fibrils were localized in indentations at the cell surface. Finally, peptides containing the arg‐gly‐asp‐ser sequence inhibited gel contraction.
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U2 - 10.1002/jcp.1041400324
DO - 10.1002/jcp.1041400324
M3 - Article
C2 - 2476450
AN - SCOPUS:0024464985
SN - 0021-9541
VL - 140
SP - 577
EP - 583
JO - Journal of cellular physiology
JF - Journal of cellular physiology
IS - 3
ER -