Abstract
Small-cell lung cancer (SCLC) is a highly malignant carcinoma with poor long-term survival. Effective treatment remains highly demanded. In the present study, we demonstrated that External Qi of Yan Xin Qigong (YXQ-EQ) exerted potent cytotoxic effect towards SCLC cell line NCI-H82 via induction of apoptosis. Global gene expression profiling identified 39 genes whose expression was altered by YXQ-EQ in NCI-82 cells. Among them, semi-quantitative RT-PCR and real-time qPCR analyses confirmed that the gene expression levels of apoptotic proteins death-associated protein kinase 2 and cell death-inducing DFFA-like effector b were upregulated, whereas that of oncoproteins DEK and MYCL1, cell migration-promoting proteins CD24 and integrin-alpha 9, and glycolytic enzyme aldolase A were downregulated. These findings suggest that YXQ-EQ may exert anticancer effect through modulating gene expression in a way that facilitates cancer cell apoptosis while represses proliferation, metastasis, and glucose metabolism.
Original language | English (US) |
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Pages (from-to) | 245-255 |
Number of pages | 11 |
Journal | Molecular and Cellular Biochemistry |
Volume | 363 |
Issue number | 1-2 |
DOIs | |
State | Published - Apr 2012 |
Keywords
- Anti-cancer effect
- Cell death
- Gene expression
- Small-cell lung cancer
ASJC Scopus subject areas
- Molecular Biology
- Clinical Biochemistry
- Cell Biology