Extended depth of focus multiphoton microscopy via incoherent pulse splitting

Bingying Chen, Tonmoy Chakraborty, Stephan Daetwyler, James D. Manton, Kevin Dean, Reto Fiolka

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


We present a beam splitter mask that can be easily added to a multiphoton raster scanning microscope to extend the depth of focus five-fold at a small loss in lateral resolution. The method is designed for ultrafast laser pulses or other light-sources featuring a low coherence length. In contrast to other methods of focus extension, our approach uniquely combines low complexity, high light-throughput and multicolor capability. We characterize the point spread function in a two-photon microscope and demonstrate fluorescence imaging of GFP labeled neurons in fixed brain samples as imaged with conventional and extended depth of focus two-photon microscopy.

Original languageEnglish (US)
Pages (from-to)3830-3842
Number of pages13
JournalBiomedical Optics Express
Issue number7
StatePublished - Jul 1 2020

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics


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