Abstract
We present a beam splitter mask that can be easily added to a multiphoton raster scanning microscope to extend the depth of focus five-fold at a small loss in lateral resolution. The method is designed for ultrafast laser pulses or other light-sources featuring a low coherence length. In contrast to other methods of focus extension, our approach uniquely combines low complexity, high light-throughput and multicolor capability. We characterize the point spread function in a two-photon microscope and demonstrate fluorescence imaging of GFP labeled neurons in fixed brain samples as imaged with conventional and extended depth of focus two-photon microscopy.
Original language | English (US) |
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Pages (from-to) | 3830-3842 |
Number of pages | 13 |
Journal | Biomedical Optics Express |
Volume | 11 |
Issue number | 7 |
DOIs | |
State | Published - Jul 1 2020 |
ASJC Scopus subject areas
- Biotechnology
- Atomic and Molecular Physics, and Optics