TY - JOUR
T1 - Expression of the tumor necrosis factor gene by dermal fibroblasts in response to ultraviolet irradiation or lipopolysaccharide
AU - De Kossodo, S.
AU - Cruz, Ponciano D
AU - Dougherty, I.
AU - Thompson, P.
AU - Silva-Valdez, M.
AU - Beutler, Bruce A
N1 - Funding Information:
S. de K. is supported by a grant from the Fonds National pour la Recherche Suisse (Switzerland). These studies used the resources of the University of Texas Southwestern Medical Center Skin Disease Research Core Center (NIH-AR-4-1940) and the University of Texas Southwestern. Diabetes Center (5-POIDK42582). We wish to thank Ms. Lesa Ellinger for expert technical assistance.
PY - 1995
Y1 - 1995
N2 - To examine the effects of different wavelengths of ultraviolet (UV) radiation on tumor necrosis factor (TNF) production, we took advantage of mice carrying a chloramphenicol acetyl transferase (CAT) reporter transgene bearing the entire TNF promoter and 3'-untranslated region. Aside from constitutive expression in the thymus, CAT activity was detected only in locally UVB- or UVC-irradiated skin. After UVB irradiation, markedly greater amounts of CAT activity were traced to the dermis rather than the epidermis; by contrast, almost all CAT activity was localized to the epidermis after UVC irradiation. Fibroblasts have not been shown previously to express the TNF gene, i.e., the TNF gene is highly methylated and inaccessible to exogenous modulation in 3T3 fibroblasts. However, the present report reveals that cultured dermal fibroblasts are capable of producing both CAT and TNF in response to treatment in vitro with either UVB irradiation, UVC irradiation, or lipopolysaccharide. These findings indicate that dermal fibroblasts may serve not only as a target for but also as a source of TNF.
AB - To examine the effects of different wavelengths of ultraviolet (UV) radiation on tumor necrosis factor (TNF) production, we took advantage of mice carrying a chloramphenicol acetyl transferase (CAT) reporter transgene bearing the entire TNF promoter and 3'-untranslated region. Aside from constitutive expression in the thymus, CAT activity was detected only in locally UVB- or UVC-irradiated skin. After UVB irradiation, markedly greater amounts of CAT activity were traced to the dermis rather than the epidermis; by contrast, almost all CAT activity was localized to the epidermis after UVC irradiation. Fibroblasts have not been shown previously to express the TNF gene, i.e., the TNF gene is highly methylated and inaccessible to exogenous modulation in 3T3 fibroblasts. However, the present report reveals that cultured dermal fibroblasts are capable of producing both CAT and TNF in response to treatment in vitro with either UVB irradiation, UVC irradiation, or lipopolysaccharide. These findings indicate that dermal fibroblasts may serve not only as a target for but also as a source of TNF.
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U2 - 10.1111/1523-1747.ep12665361
DO - 10.1111/1523-1747.ep12665361
M3 - Article
C2 - 7860994
AN - SCOPUS:0028926559
SN - 0022-202X
VL - 104
SP - 318
EP - 322
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 3
ER -