Expression of the E. coli Lac Z gene from a defective HSV-1 vector in various human normal, cancer-prone and tumor cells

David A. Boothman; Alfred I. Geller; Arthur B. Pardee

doi:10.1016/0014-5793(89)81640-0

Expression of the E. coli Lac Z gene from a defective HSV-1 vector in various human normal, cancer-prone and tumor cells

David A. Boothman, Alfred I. Geller, Arthur B. Pardee

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Introducing foreign genetic material into human cells is essential for the elucidation of the function of various human genes and has potential use in the treatment of human diseases by gene therapy. In this study we demonstrate that a defective herpes simplex virus type 1 vector, pHSVlac, can effectively transfer and express the Escherichia coli Lac Z gene in a variety of exponential and quiescent human cells. The human cells tested included representative cells derived from cancer-prone patients that presumably have various DNA repair deficiencies.

Original language	English (US)
Pages (from-to)	159-162
Number of pages	4
Journal	FEBS Letters
Volume	258
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(89)81640-0
State	Published - Nov 20 1989

Keywords

DNA damage, potentially lethal
DNA repair
Gene therapy
HSV-1 vector, defective
Host range

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(89)81640-0

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title = "Expression of the E. coli Lac Z gene from a defective HSV-1 vector in various human normal, cancer-prone and tumor cells",

abstract = "Introducing foreign genetic material into human cells is essential for the elucidation of the function of various human genes and has potential use in the treatment of human diseases by gene therapy. In this study we demonstrate that a defective herpes simplex virus type 1 vector, pHSVlac, can effectively transfer and express the Escherichia coli Lac Z gene in a variety of exponential and quiescent human cells. The human cells tested included representative cells derived from cancer-prone patients that presumably have various DNA repair deficiencies.",

keywords = "DNA damage, potentially lethal, DNA repair, Gene therapy, HSV-1 vector, defective, Host range",

author = "Boothman, {David A.} and Geller, {Alfred I.} and Pardee, {Arthur B.}",

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T1 - Expression of the E. coli Lac Z gene from a defective HSV-1 vector in various human normal, cancer-prone and tumor cells

AU - Boothman, David A.

AU - Geller, Alfred I.

AU - Pardee, Arthur B.

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PY - 1989/11/20

Y1 - 1989/11/20

N2 - Introducing foreign genetic material into human cells is essential for the elucidation of the function of various human genes and has potential use in the treatment of human diseases by gene therapy. In this study we demonstrate that a defective herpes simplex virus type 1 vector, pHSVlac, can effectively transfer and express the Escherichia coli Lac Z gene in a variety of exponential and quiescent human cells. The human cells tested included representative cells derived from cancer-prone patients that presumably have various DNA repair deficiencies.

AB - Introducing foreign genetic material into human cells is essential for the elucidation of the function of various human genes and has potential use in the treatment of human diseases by gene therapy. In this study we demonstrate that a defective herpes simplex virus type 1 vector, pHSVlac, can effectively transfer and express the Escherichia coli Lac Z gene in a variety of exponential and quiescent human cells. The human cells tested included representative cells derived from cancer-prone patients that presumably have various DNA repair deficiencies.

KW - DNA damage, potentially lethal

KW - DNA repair

KW - Gene therapy

KW - HSV-1 vector, defective

KW - Host range

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Expression of the E. coli Lac Z gene from a defective HSV-1 vector in various human normal, cancer-prone and tumor cells

Abstract

Keywords

ASJC Scopus subject areas

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