TY - JOUR
T1 - Exploration of Nanoparticle-Mediated Photothermal Effect of TMB-H2O2 Colorimetric System and Its Application in a Visual Quantitative Photothermal Immunoassay
AU - Fu, Guanglei
AU - Sanjay, Sharma T.
AU - Zhou, Wan
AU - Brekken, Rolf A.
AU - Kirken, Robert A.
AU - Li, Xiujun
N1 - Funding Information:
We are grateful for the financial support from the National Institute of Allergy and Infectious Disease of the NIH (R21AI107415), the National Institute of General Medical Sciences of the NIH (SC2GM105584), and the U.S. NSF-PREM Program (DMR 1205302). Financial support from the Emily Koenig Meningitis Fund and Philadelphia Foundation, the NIH RCMI Pilot Grant, the Medical Center of the Americas Foundation, the NIH BUILDing Scholar Summer Sabbatical Award (NIGMS Award numbers RL5GM118969, TL4GM118971, and UL1GM11897), the University of Texas at El Paso for the IDR Program, and the University of Texas system for the STARS Award is also gratefully acknowledged. We are also grateful to Mr. Jeffrey Gonzalez and Dave Primm for help in the manuscript preparation.
Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/5/1
Y1 - 2018/5/1
N2 - The exploration of new physical and chemical properties of materials and their innovative application in different fields are of great importance to advance analytical chemistry, material science, and other important fields. Herein, we, for the first time, discovered the photothermal effect of an iron oxide nanoparticles (NPs)-mediated TMB (3,3′,5,5′-tetramethylbenzidine)-H2O2 colorimetric system, and applied it toward the development of a new NP-mediated photothermal immunoassay platform for visual quantitative biomolecule detection using a thermometer as the signal reader. Using a sandwich-type proof-of-concept immunoassay, we found that the charge transfer complex of the iron oxide NPs-mediated one-electron oxidation product of TMB (oxidized TMB) exhibited not only color changes, but also a strong near-infrared (NIR) laser-driven photothermal effect. Hence, oxidized TMB was explored as a new sensitive photothermal probe to convert the immunoassay signal into heat through the near-infrared laser-driven photothermal effect, enabling simple photothermal immunoassay using a thermometer. Based on the new iron oxide NPs-mediated TMB-H2O2 photothermal immunoassay platform, prostate-specific antigen (PSA) as a model biomarker can be detected at a concentration as low as 1.0 ng·mL-1 in normal human serum. The discovered photothermal effect of the colorimetric system and the developed new photothermal immunoassay platform open up a new horizon for affordable detection of disease biomarkers and have great potential for other important material and biomedical applications of interest.
AB - The exploration of new physical and chemical properties of materials and their innovative application in different fields are of great importance to advance analytical chemistry, material science, and other important fields. Herein, we, for the first time, discovered the photothermal effect of an iron oxide nanoparticles (NPs)-mediated TMB (3,3′,5,5′-tetramethylbenzidine)-H2O2 colorimetric system, and applied it toward the development of a new NP-mediated photothermal immunoassay platform for visual quantitative biomolecule detection using a thermometer as the signal reader. Using a sandwich-type proof-of-concept immunoassay, we found that the charge transfer complex of the iron oxide NPs-mediated one-electron oxidation product of TMB (oxidized TMB) exhibited not only color changes, but also a strong near-infrared (NIR) laser-driven photothermal effect. Hence, oxidized TMB was explored as a new sensitive photothermal probe to convert the immunoassay signal into heat through the near-infrared laser-driven photothermal effect, enabling simple photothermal immunoassay using a thermometer. Based on the new iron oxide NPs-mediated TMB-H2O2 photothermal immunoassay platform, prostate-specific antigen (PSA) as a model biomarker can be detected at a concentration as low as 1.0 ng·mL-1 in normal human serum. The discovered photothermal effect of the colorimetric system and the developed new photothermal immunoassay platform open up a new horizon for affordable detection of disease biomarkers and have great potential for other important material and biomedical applications of interest.
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U2 - 10.1021/acs.analchem.8b00842
DO - 10.1021/acs.analchem.8b00842
M3 - Article
C2 - 29641893
AN - SCOPUS:85046411023
SN - 0003-2700
VL - 90
SP - 5930
EP - 5937
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 9
ER -