TY - JOUR
T1 - Experimental hyperphenylalaninemia
T2 - Dendritic alterations in motor cortex of rat
AU - Hogan, R. N.
AU - Coleman, P. D.
N1 - Funding Information:
Abbreviations: HPA-hyperphenylalaninemia, PKU-phenylketonuria, nine, PheH-phenylalanine hydroxylase, PCPA-para-chlorophenylalanine. ’ A preliminary report of these findings was presented at the VIlIth International Congress of Neuropathology in Washington, D.C., June 1978. The authors wish to thank A. LeBlanc and Dr. D. Flood for their technical assistance and Dr. N. Malamud, Dr. D. Flood, and Dr. H. Panitch for reading the manuscript. Supported by National Institutes of Health grant AGI 121. Reprint requests should be sent to Dr. Hogan’s current address: Department of Neurology, Veterans Administration Medical Center, 4150 Clement St., San Francisco, CA 94121.
PY - 1981/10
Y1 - 1981/10
N2 - Rats were injected i.p. daily from postnatal days 3 through 82 with a combination of the phenylalanine hydroxylase inhibitor p-chlorophenylalanine (60 mg/kg) plus phenylalanine (300 mg/kg), resulting in a significant hyperphenylalaninemia of 5.36 mg 100 ml. Control rats were saline injected. Golgi-Cox-stained layer V pyramidal and layer IV stellate neurons from cortical area 4 were measured using a computerized dendrite tracking system. Basal and oblique dendritic segments of layer V pyramidal cells in animals with hyperphenylalaninemia were fewer in number, shorter, branched closer to the cell soma or apical shaft, and branched less frequently than did dendrites in control animals. Fewer differences were found for layer IV stellate cells. These data suggest that in human hyperphenylalaninemia which is not severe enough to satisfy the classification criterion for phenylketonuria of 16.5 mg 100 ml plasma there may be undetected alterations in the morphology of cortical dendrites.
AB - Rats were injected i.p. daily from postnatal days 3 through 82 with a combination of the phenylalanine hydroxylase inhibitor p-chlorophenylalanine (60 mg/kg) plus phenylalanine (300 mg/kg), resulting in a significant hyperphenylalaninemia of 5.36 mg 100 ml. Control rats were saline injected. Golgi-Cox-stained layer V pyramidal and layer IV stellate neurons from cortical area 4 were measured using a computerized dendrite tracking system. Basal and oblique dendritic segments of layer V pyramidal cells in animals with hyperphenylalaninemia were fewer in number, shorter, branched closer to the cell soma or apical shaft, and branched less frequently than did dendrites in control animals. Fewer differences were found for layer IV stellate cells. These data suggest that in human hyperphenylalaninemia which is not severe enough to satisfy the classification criterion for phenylketonuria of 16.5 mg 100 ml plasma there may be undetected alterations in the morphology of cortical dendrites.
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U2 - 10.1016/0014-4886(81)90160-6
DO - 10.1016/0014-4886(81)90160-6
M3 - Article
C2 - 6456925
AN - SCOPUS:0019486014
SN - 0014-4886
VL - 74
SP - 218
EP - 233
JO - Experimental Neurology
JF - Experimental Neurology
IS - 1
ER -