TY - JOUR
T1 - Evidence that tissue recoil in the early Drosophila embryo is a passive not active process
AU - Goldner, Amanda Nicole
AU - Fessehaye, Salena M.
AU - Rodriguez, Nataly
AU - Mapes, Kelly Ann
AU - Osterfield, Miriam
AU - Doubrovinski, Konstantin
N1 - Publisher Copyright:
© 2023 Goldner et al.
PY - 2023/9/1
Y1 - 2023/9/1
N2 - Understanding tissue morphogenesis is impossible without knowing the mechanical properties of the tissue being shaped. Although techniques for measuring tissue material properties are continually being developed, methods for determining how individual proteins contribute to mechanical properties are very limited. Here, we developed two complementary techniques for the acute inactivation of spaghetti squash (the Drosophila myosin regulatory light chain), one based on the recently introduced (auxin-inducible degron 2 (AID2) system, and the other based on a novel method for conditional protein aggregation that results in nearly instantaneous protein inactivation. Combining these techniques with rheological measurements, we show that passive material properties of the cellularization-stage Drosophila embryo are essentially unaffected by myosin activity. These results suggest that this tissue is elastic, not predominantly viscous, on the developmentally relevant timescale.
AB - Understanding tissue morphogenesis is impossible without knowing the mechanical properties of the tissue being shaped. Although techniques for measuring tissue material properties are continually being developed, methods for determining how individual proteins contribute to mechanical properties are very limited. Here, we developed two complementary techniques for the acute inactivation of spaghetti squash (the Drosophila myosin regulatory light chain), one based on the recently introduced (auxin-inducible degron 2 (AID2) system, and the other based on a novel method for conditional protein aggregation that results in nearly instantaneous protein inactivation. Combining these techniques with rheological measurements, we show that passive material properties of the cellularization-stage Drosophila embryo are essentially unaffected by myosin activity. These results suggest that this tissue is elastic, not predominantly viscous, on the developmentally relevant timescale.
UR - http://www.scopus.com/inward/record.url?scp=85168245328&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85168245328&partnerID=8YFLogxK
U2 - 10.1091/mbc.E22-09-0409
DO - 10.1091/mbc.E22-09-0409
M3 - Article
C2 - 37405768
AN - SCOPUS:85168245328
SN - 1059-1524
VL - 34
JO - Molecular biology of the cell
JF - Molecular biology of the cell
IS - 10
M1 - 0409
ER -