Evaluation of triple quantum-filtered 23Na NMR spectroscopy in the in situ rat liver

Viswanathan Seshan; A. Dean Sherry; Navin Bansal

doi:10.1002/mrm.1910380519

Evaluation of triple quantum-filtered ²³Na NMR spectroscopy in the in situ rat liver

Viswanathan Seshan, A. Dean Sherry, Navin Bansal

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Triple quantum (TQ)-filtered ²³Na NMR spectroscopy and the shift reagent, TmDOTP^5-, have been used to evaluate the contributions of intra- (Na(i)⁺) and extracellular (Na(e)⁺) sodium to the TQ-filtered signal in the rat liver, in situ. Na(e)⁺ contributed significantly to the total TQ- filtered signal in live animals, and the intensity of this signal did not change postmortem. The TQ-filtered Na(i)⁺ signal increased by approximately 380% over a period of 1 h postmortem, whereas the single quantum (SQ) Na(i)⁺ increased by 90%. The constancy of the TQ-filtered Na(e)⁺ signal indicates that changes in total TQ-filtered ²³Na signal intensity in liver (without a shift reagent) may accurately reflect changes in TQ-filtered Na(i)⁺ signal intensity. The large percent increase in the TQ-filtered Na(i)⁺ signal as compared to the SQ signal suggests that the fraction of Na(i)⁺ interacting with macromolecules increases after death.

Original language	English (US)
Pages (from-to)	821-827
Number of pages	7
Journal	Magnetic resonance in medicine
Volume	38
Issue number	5
DOIs	https://doi.org/10.1002/mrm.1910380519
State	Published - Nov 1997

Keywords

Liver
Multiple quantum NMR
Na MRS
Shift reagent

ASJC Scopus subject areas

Radiology Nuclear Medicine and imaging

Access to Document

10.1002/mrm.1910380519

Cite this

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title = "Evaluation of triple quantum-filtered 23Na NMR spectroscopy in the in situ rat liver",

abstract = "Triple quantum (TQ)-filtered 23Na NMR spectroscopy and the shift reagent, TmDOTP5-, have been used to evaluate the contributions of intra- (Na(i)+) and extracellular (Na(e)+) sodium to the TQ-filtered signal in the rat liver, in situ. Na(e)+ contributed significantly to the total TQ- filtered signal in live animals, and the intensity of this signal did not change postmortem. The TQ-filtered Na(i)+ signal increased by approximately 380% over a period of 1 h postmortem, whereas the single quantum (SQ) Na(i)+ increased by 90%. The constancy of the TQ-filtered Na(e)+ signal indicates that changes in total TQ-filtered 23Na signal intensity in liver (without a shift reagent) may accurately reflect changes in TQ-filtered Na(i)+ signal intensity. The large percent increase in the TQ-filtered Na(i)+ signal as compared to the SQ signal suggests that the fraction of Na(i)+ interacting with macromolecules increases after death.",

keywords = "Liver, Multiple quantum NMR, Na MRS, Shift reagent",

author = "Viswanathan Seshan and Sherry, {A. Dean} and Navin Bansal",

year = "1997",

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journal = "Magnetic resonance in medicine",

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T1 - Evaluation of triple quantum-filtered 23Na NMR spectroscopy in the in situ rat liver

AU - Seshan, Viswanathan

AU - Sherry, A. Dean

AU - Bansal, Navin

PY - 1997/11

Y1 - 1997/11

N2 - Triple quantum (TQ)-filtered 23Na NMR spectroscopy and the shift reagent, TmDOTP5-, have been used to evaluate the contributions of intra- (Na(i)+) and extracellular (Na(e)+) sodium to the TQ-filtered signal in the rat liver, in situ. Na(e)+ contributed significantly to the total TQ- filtered signal in live animals, and the intensity of this signal did not change postmortem. The TQ-filtered Na(i)+ signal increased by approximately 380% over a period of 1 h postmortem, whereas the single quantum (SQ) Na(i)+ increased by 90%. The constancy of the TQ-filtered Na(e)+ signal indicates that changes in total TQ-filtered 23Na signal intensity in liver (without a shift reagent) may accurately reflect changes in TQ-filtered Na(i)+ signal intensity. The large percent increase in the TQ-filtered Na(i)+ signal as compared to the SQ signal suggests that the fraction of Na(i)+ interacting with macromolecules increases after death.

AB - Triple quantum (TQ)-filtered 23Na NMR spectroscopy and the shift reagent, TmDOTP5-, have been used to evaluate the contributions of intra- (Na(i)+) and extracellular (Na(e)+) sodium to the TQ-filtered signal in the rat liver, in situ. Na(e)+ contributed significantly to the total TQ- filtered signal in live animals, and the intensity of this signal did not change postmortem. The TQ-filtered Na(i)+ signal increased by approximately 380% over a period of 1 h postmortem, whereas the single quantum (SQ) Na(i)+ increased by 90%. The constancy of the TQ-filtered Na(e)+ signal indicates that changes in total TQ-filtered 23Na signal intensity in liver (without a shift reagent) may accurately reflect changes in TQ-filtered Na(i)+ signal intensity. The large percent increase in the TQ-filtered Na(i)+ signal as compared to the SQ signal suggests that the fraction of Na(i)+ interacting with macromolecules increases after death.

KW - Liver

KW - Multiple quantum NMR

KW - Na MRS

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