@inbook{058e31792cbf4682988b84414a63716f,
title = "Evaluating Melanoma Viability and Proliferation in 3D Microenvironments",
abstract = "Researchers often aim to incorporate microenvironmental variables such as the dimensionality and composition of the extracellular matrix into their cell-based assays. A technical challenge created by introduction of these variables is quantification of single-cell measurements and control of environmental reproducibility. Here, we detail a methodology to quantify viability and proliferation of melanoma cells in 3D collagen-based culture platforms by automated microscopy and 3D image analysis to yield robust, high-throughput results of single-cell responses to drug treatment.",
keywords = "3D cell culture, Collagen matrix, Drug screening, Extracellular matrix, Image analysis",
author = "{Siruvallur Murali}, Vasanth and Cobanoglu, {Murat Can} and Welf, {Erik S.}",
note = "Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1205-7_12",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "155--171",
booktitle = "Methods in Molecular Biology",
}