Evaluating Melanoma Viability and Proliferation in 3D Microenvironments

Vasanth Siruvallur Murali, Murat Can Cobanoglu, Erik S. Welf

Research output: Chapter in Book/Report/Conference proceedingChapter


Researchers often aim to incorporate microenvironmental variables such as the dimensionality and composition of the extracellular matrix into their cell-based assays. A technical challenge created by introduction of these variables is quantification of single-cell measurements and control of environmental reproducibility. Here, we detail a methodology to quantify viability and proliferation of melanoma cells in 3D collagen-based culture platforms by automated microscopy and 3D image analysis to yield robust, high-throughput results of single-cell responses to drug treatment.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages17
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • 3D cell culture
  • Collagen matrix
  • Drug screening
  • Extracellular matrix
  • Image analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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