ETB receptor activation causes exocytic insertion of NHE3 in OKP cells

Yan Peng, Morimasa Amemiya, Xiaojing Yang, Lingzhi Fan, Orson W Moe, Helen L Yin, Patricia A. Preisig, Masashi Yanagisawa, Robert J. Alpern

Research output: Contribution to journalArticlepeer-review

68 Scopus citations


Endothelin-1 (ET-1) activates sodium/hydrogen exchanger 3 (NHE3) in opossum kidney clone P (OKP) cells expressing ETB receptors. ET-1 (10-8 M) caused a two- to threefold increase in apical membrane NHE3 (assessed by surface biotinylation), in the absence of a change in total cellular NHE3. A maximal effect was achieved within 15 min. The increase in apical NHE3 was not blocked by cytochalasin D but was blocked by latrunculin B, which also prevented the ET-1-induced increase in NHE3 activity. Endocytic internalization of NHE3, measured as protection of biotinylated NHE3 from the membrane-impermeant, sulfhydryl-reducing agent MesNa was minimal within 35 min and was not regulated by ET-1. Exocytic insertion of NHE3, measured as the appearance of biotinylated NHE3 after the blockade of reactive sites with sulfo-NHS-acetate, was increased in response to ET-1. These studies demonstrate that ET-1 induces net trafficking of NHE3 to the apical membrane that is mediated by enhanced exocytic insertion and is required for increased NHE3 activity.

Original languageEnglish (US)
Pages (from-to)F34-F42
JournalAmerican Journal of Physiology - Renal Physiology
Issue number1 49-1
StatePublished - Jan 2001


  • Endothelin
  • Membrane trafficking
  • Opossum kidney clone 3
  • Proximal tubule
  • Sodium/hydrogen antiporter
  • Sodium/hydrogen exchanger 3

ASJC Scopus subject areas

  • Physiology
  • Urology


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