Establishment of bovine trophoblast stem cells

Yinjuan Wang, Hao Ming, Leqian Yu, Jie Li, Linkai Zhu, Hai Xi Sun, Carlos A. Pinzon-Arteaga, Jun Wu, Zongliang Jiang

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Here, we report that a chemical cocktail (LCDM: leukemia inhibitory factor [LIF], CHIR99021, dimethinedene maleate [DiM], minocycline hydrochloride), previously developed for extended pluripotent stem cells (EPSCs) in mice and humans, enables de novo derivation and long-term culture of bovine trophoblast stem cells (TSCs). Bovine TSCs retain developmental potency to differentiate into mature trophoblast cells and exhibit transcriptomic and epigenetic (chromatin accessibility and DNA methylome) features characteristic of trophectoderm cells from early bovine embryos. The bovine TSCs established in this study will provide a model to study bovine placentation and early pregnancy failure.

Original languageEnglish (US)
Article number112439
JournalCell Reports
Volume42
Issue number5
DOIs
StatePublished - May 30 2023

Keywords

  • CP: Stem cell research
  • blastocyst
  • bovine
  • pluripotency
  • trophectoderm
  • trophoblast differentiation
  • trophoblast stem cells

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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