Establishment of bovine trophoblast stem cells

Yinjuan Wang; Hao Ming; Leqian Yu; Jie Li; Linkai Zhu; Hai Xi Sun; Carlos A. Pinzon-Arteaga; Jun Wu; Zongliang Jiang

doi:10.1016/j.celrep.2023.112439

Establishment of bovine trophoblast stem cells

Yinjuan Wang, Hao Ming, Leqian Yu, Jie Li, Linkai Zhu, Hai Xi Sun, Carlos A. Pinzon-Arteaga, Jun Wu, Zongliang Jiang

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Here, we report that a chemical cocktail (LCDM: leukemia inhibitory factor [LIF], CHIR99021, dimethinedene maleate [DiM], minocycline hydrochloride), previously developed for extended pluripotent stem cells (EPSCs) in mice and humans, enables de novo derivation and long-term culture of bovine trophoblast stem cells (TSCs). Bovine TSCs retain developmental potency to differentiate into mature trophoblast cells and exhibit transcriptomic and epigenetic (chromatin accessibility and DNA methylome) features characteristic of trophectoderm cells from early bovine embryos. The bovine TSCs established in this study will provide a model to study bovine placentation and early pregnancy failure.

Original language	English (US)
Article number	112439
Journal	Cell Reports
Volume	42
Issue number	5
DOIs	https://doi.org/10.1016/j.celrep.2023.112439
State	Published - May 30 2023

Keywords

CP: Stem cell research
blastocyst
bovine
pluripotency
trophectoderm
trophoblast differentiation
trophoblast stem cells

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.celrep.2023.112439

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title = "Establishment of bovine trophoblast stem cells",

abstract = "Here, we report that a chemical cocktail (LCDM: leukemia inhibitory factor [LIF], CHIR99021, dimethinedene maleate [DiM], minocycline hydrochloride), previously developed for extended pluripotent stem cells (EPSCs) in mice and humans, enables de novo derivation and long-term culture of bovine trophoblast stem cells (TSCs). Bovine TSCs retain developmental potency to differentiate into mature trophoblast cells and exhibit transcriptomic and epigenetic (chromatin accessibility and DNA methylome) features characteristic of trophectoderm cells from early bovine embryos. The bovine TSCs established in this study will provide a model to study bovine placentation and early pregnancy failure.",

keywords = "CP: Stem cell research, blastocyst, bovine, pluripotency, trophectoderm, trophoblast differentiation, trophoblast stem cells",

author = "Yinjuan Wang and Hao Ming and Leqian Yu and Jie Li and Linkai Zhu and Sun, {Hai Xi} and Pinzon-Arteaga, {Carlos A.} and Jun Wu and Zongliang Jiang",

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doi = "10.1016/j.celrep.2023.112439",

language = "English (US)",

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T1 - Establishment of bovine trophoblast stem cells

AU - Wang, Yinjuan

AU - Ming, Hao

AU - Yu, Leqian

AU - Li, Jie

AU - Zhu, Linkai

AU - Sun, Hai Xi

AU - Pinzon-Arteaga, Carlos A.

AU - Wu, Jun

AU - Jiang, Zongliang

PY - 2023/5/30

Y1 - 2023/5/30

N2 - Here, we report that a chemical cocktail (LCDM: leukemia inhibitory factor [LIF], CHIR99021, dimethinedene maleate [DiM], minocycline hydrochloride), previously developed for extended pluripotent stem cells (EPSCs) in mice and humans, enables de novo derivation and long-term culture of bovine trophoblast stem cells (TSCs). Bovine TSCs retain developmental potency to differentiate into mature trophoblast cells and exhibit transcriptomic and epigenetic (chromatin accessibility and DNA methylome) features characteristic of trophectoderm cells from early bovine embryos. The bovine TSCs established in this study will provide a model to study bovine placentation and early pregnancy failure.

AB - Here, we report that a chemical cocktail (LCDM: leukemia inhibitory factor [LIF], CHIR99021, dimethinedene maleate [DiM], minocycline hydrochloride), previously developed for extended pluripotent stem cells (EPSCs) in mice and humans, enables de novo derivation and long-term culture of bovine trophoblast stem cells (TSCs). Bovine TSCs retain developmental potency to differentiate into mature trophoblast cells and exhibit transcriptomic and epigenetic (chromatin accessibility and DNA methylome) features characteristic of trophectoderm cells from early bovine embryos. The bovine TSCs established in this study will provide a model to study bovine placentation and early pregnancy failure.

KW - CP: Stem cell research

KW - blastocyst

KW - bovine

KW - pluripotency

KW - trophectoderm

KW - trophoblast differentiation

KW - trophoblast stem cells

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JO - Cell Reports

JF - Cell Reports

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Establishment of bovine trophoblast stem cells

Abstract

Keywords

ASJC Scopus subject areas

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