Abstract
Here, we report that a chemical cocktail (LCDM: leukemia inhibitory factor [LIF], CHIR99021, dimethinedene maleate [DiM], minocycline hydrochloride), previously developed for extended pluripotent stem cells (EPSCs) in mice and humans, enables de novo derivation and long-term culture of bovine trophoblast stem cells (TSCs). Bovine TSCs retain developmental potency to differentiate into mature trophoblast cells and exhibit transcriptomic and epigenetic (chromatin accessibility and DNA methylome) features characteristic of trophectoderm cells from early bovine embryos. The bovine TSCs established in this study will provide a model to study bovine placentation and early pregnancy failure.
Original language | English (US) |
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Article number | 112439 |
Journal | Cell Reports |
Volume | 42 |
Issue number | 5 |
DOIs | |
State | Published - May 30 2023 |
Keywords
- CP: Stem cell research
- blastocyst
- bovine
- pluripotency
- trophectoderm
- trophoblast differentiation
- trophoblast stem cells
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology