Epstein-Barr virus EBER transcripts affect miRNA-mediated regulation of specific targets and are processed to small RNA species

Julia Alles; Daniele Hasler; Syed Mohammad Ali Kazmi; Mathias Tesson; Andrew Hamilton; Linda Schlegel; Stefanie Marx; Norbert Eichner; Richard Reinhardt; Gunter Meister; Joanna B. Wilson; Friedrich A. Grässer

doi:10.3390/ncrna1030170

Epstein-Barr virus EBER transcripts affect miRNA-mediated regulation of specific targets and are processed to small RNA species

Julia Alles, Daniele Hasler, Syed Mohammad Ali Kazmi, Mathias Tesson, Andrew Hamilton, Linda Schlegel, Stefanie Marx, Norbert Eichner, Richard Reinhardt, Gunter Meister, Joanna B. Wilson, Friedrich A. Grässer

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The oncogenic Epstein-Barr virus (EBV) expresses 44 mature microRNAs and two non-coding EBER RNAs of 167 (EBER1) and 172 (EBER2) nt length. MiRNA profiling of NK/T cell lines and primary cells and Northern blotting of EBV-infected cell lines and primary tumors revealed processing of EBER1 to short 5'-derived RNAs of approximately 23, 52 and 70 nt (EBER1₂₃, EBER1₅₂, and EBER1₇₀) and of EBER2 to 3' fragments. The biogenesis of these species is independent of Dicer, and EBER1₂₃ does not act like a miRNA to target its complementary sequence. EBER1, EBER2 and EBER1₂₃ were bound by the lupus antigen (La), a nuclear and cytoplasmic protein that facilitates RNAi. Consistent with this, the EBERs affect regulation of interleukin 1alpha (IL1α) and RAC1 reporters harboring miR target sequences, targets of miR-142-3p. However, the EBERs have no effect upon another target of miR-142-3p, ADCY9, nor on TOMM22, a target of ebv-miR-BART16, indicative of selective modulation of gene expression by the EBERs.

Original language	English (US)
Pages (from-to)	170-191
Number of pages	22
Journal	Non-coding RNA
Volume	1
Issue number	3
DOIs	https://doi.org/10.3390/ncrna1030170
State	Published - Sep 30 2015
Externally published	Yes

Keywords

ADCY9
DICER
EBER
EBV
Ebv-miR-BART16
Epstein-Barr virus
Hsa-miR-142
IL1A
La
Lupus antigen
MicroRNA
RAC1
TOMM22

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Genetics

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10.3390/ncrna1030170

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title = "Epstein-Barr virus EBER transcripts affect miRNA-mediated regulation of specific targets and are processed to small RNA species",

abstract = "The oncogenic Epstein-Barr virus (EBV) expresses 44 mature microRNAs and two non-coding EBER RNAs of 167 (EBER1) and 172 (EBER2) nt length. MiRNA profiling of NK/T cell lines and primary cells and Northern blotting of EBV-infected cell lines and primary tumors revealed processing of EBER1 to short 5'-derived RNAs of approximately 23, 52 and 70 nt (EBER123, EBER152, and EBER170) and of EBER2 to 3' fragments. The biogenesis of these species is independent of Dicer, and EBER123 does not act like a miRNA to target its complementary sequence. EBER1, EBER2 and EBER123 were bound by the lupus antigen (La), a nuclear and cytoplasmic protein that facilitates RNAi. Consistent with this, the EBERs affect regulation of interleukin 1alpha (IL1α) and RAC1 reporters harboring miR target sequences, targets of miR-142-3p. However, the EBERs have no effect upon another target of miR-142-3p, ADCY9, nor on TOMM22, a target of ebv-miR-BART16, indicative of selective modulation of gene expression by the EBERs.",

keywords = "ADCY9, DICER, EBER, EBV, Ebv-miR-BART16, Epstein-Barr virus, Hsa-miR-142, IL1A, La, Lupus antigen, MicroRNA, RAC1, TOMM22",

author = "Julia Alles and Daniele Hasler and {Ali Kazmi}, {Syed Mohammad} and Mathias Tesson and Andrew Hamilton and Linda Schlegel and Stefanie Marx and Norbert Eichner and Richard Reinhardt and Gunter Meister and Wilson, {Joanna B.} and Gr{\"a}sser, {Friedrich A.}",

note = "Publisher Copyright: {\textcopyright} 2015 by the authors.",

year = "2015",

month = sep,

day = "30",

doi = "10.3390/ncrna1030170",

language = "English (US)",

volume = "1",

pages = "170--191",

journal = "Non-coding RNA",

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publisher = "MDPI AG",

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TY - JOUR

T1 - Epstein-Barr virus EBER transcripts affect miRNA-mediated regulation of specific targets and are processed to small RNA species

AU - Alles, Julia

AU - Hasler, Daniele

AU - Ali Kazmi, Syed Mohammad

AU - Tesson, Mathias

AU - Hamilton, Andrew

AU - Schlegel, Linda

AU - Marx, Stefanie

AU - Eichner, Norbert

AU - Reinhardt, Richard

AU - Meister, Gunter

AU - Wilson, Joanna B.

AU - Grässer, Friedrich A.

PY - 2015/9/30

Y1 - 2015/9/30

N2 - The oncogenic Epstein-Barr virus (EBV) expresses 44 mature microRNAs and two non-coding EBER RNAs of 167 (EBER1) and 172 (EBER2) nt length. MiRNA profiling of NK/T cell lines and primary cells and Northern blotting of EBV-infected cell lines and primary tumors revealed processing of EBER1 to short 5'-derived RNAs of approximately 23, 52 and 70 nt (EBER123, EBER152, and EBER170) and of EBER2 to 3' fragments. The biogenesis of these species is independent of Dicer, and EBER123 does not act like a miRNA to target its complementary sequence. EBER1, EBER2 and EBER123 were bound by the lupus antigen (La), a nuclear and cytoplasmic protein that facilitates RNAi. Consistent with this, the EBERs affect regulation of interleukin 1alpha (IL1α) and RAC1 reporters harboring miR target sequences, targets of miR-142-3p. However, the EBERs have no effect upon another target of miR-142-3p, ADCY9, nor on TOMM22, a target of ebv-miR-BART16, indicative of selective modulation of gene expression by the EBERs.

AB - The oncogenic Epstein-Barr virus (EBV) expresses 44 mature microRNAs and two non-coding EBER RNAs of 167 (EBER1) and 172 (EBER2) nt length. MiRNA profiling of NK/T cell lines and primary cells and Northern blotting of EBV-infected cell lines and primary tumors revealed processing of EBER1 to short 5'-derived RNAs of approximately 23, 52 and 70 nt (EBER123, EBER152, and EBER170) and of EBER2 to 3' fragments. The biogenesis of these species is independent of Dicer, and EBER123 does not act like a miRNA to target its complementary sequence. EBER1, EBER2 and EBER123 were bound by the lupus antigen (La), a nuclear and cytoplasmic protein that facilitates RNAi. Consistent with this, the EBERs affect regulation of interleukin 1alpha (IL1α) and RAC1 reporters harboring miR target sequences, targets of miR-142-3p. However, the EBERs have no effect upon another target of miR-142-3p, ADCY9, nor on TOMM22, a target of ebv-miR-BART16, indicative of selective modulation of gene expression by the EBERs.

KW - ADCY9

KW - DICER

KW - EBER

KW - EBV

KW - Ebv-miR-BART16

KW - Epstein-Barr virus

KW - Hsa-miR-142

KW - IL1A

KW - La

KW - Lupus antigen

KW - MicroRNA

KW - RAC1

KW - TOMM22

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U2 - 10.3390/ncrna1030170

DO - 10.3390/ncrna1030170

M3 - Article

C2 - 29861423

AN - SCOPUS:84962126244

SN - 2311-553X

VL - 1

SP - 170

EP - 191

JO - Non-coding RNA

JF - Non-coding RNA

IS - 3

ER -

Epstein-Barr virus EBER transcripts affect miRNA-mediated regulation of specific targets and are processed to small RNA species

Abstract

Keywords

ASJC Scopus subject areas

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