TY - JOUR
T1 - EphB receptor forward signaling regulates area-specific reciprocal thalamic and cortical axon pathfinding
AU - Robichaux, Michael A.
AU - Chenaux, George
AU - Henry Ho, Hsin Yi
AU - Soskis, Michael J.
AU - Dravis, Christopher
AU - Kwan, Kenneth Y.
AU - Sestan, Nenad
AU - Greenberg, Michael Eldon
AU - Henkemeyer, Mark
AU - Cowan, Christopher W.
PY - 2014/2/11
Y1 - 2014/2/11
N2 - In early brain development, ascending thalamocortical axons (TCAs) navigate through the ventral telencephalon (VTel) to reach their target regions in the young cerebral cortex. Descending, deep-layer cortical axons subsequently target appropriate thalamic and subcortical target regions. However, precisely how and when corticothalamic axons (CTAs) identify their appropriate, reciprocal thalamic targets remains unclear. We show here that EphB1 and EphB2 receptors control proper navigation of a subset of TCA and CTA projections through the VTel. We show in vivo that EphB receptor forward signaling and the ephrinB1 ligand are required during the early navigation of L1-CAM+ thalamic fibers in the VTel, and that the misguided thalamic fibers in EphB1/2 KO mice appear to interact with cortical subregion-specific axon populations during reciprocal cortical axon guidance. As such, our findings suggest that descending cortical axons identify specific TCA subpopulations in the dorsal VTel to coordinate reciprocal cortical- thalamic connectivity in the early developing brain.
AB - In early brain development, ascending thalamocortical axons (TCAs) navigate through the ventral telencephalon (VTel) to reach their target regions in the young cerebral cortex. Descending, deep-layer cortical axons subsequently target appropriate thalamic and subcortical target regions. However, precisely how and when corticothalamic axons (CTAs) identify their appropriate, reciprocal thalamic targets remains unclear. We show here that EphB1 and EphB2 receptors control proper navigation of a subset of TCA and CTA projections through the VTel. We show in vivo that EphB receptor forward signaling and the ephrinB1 ligand are required during the early navigation of L1-CAM+ thalamic fibers in the VTel, and that the misguided thalamic fibers in EphB1/2 KO mice appear to interact with cortical subregion-specific axon populations during reciprocal cortical axon guidance. As such, our findings suggest that descending cortical axons identify specific TCA subpopulations in the dorsal VTel to coordinate reciprocal cortical- thalamic connectivity in the early developing brain.
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U2 - 10.1073/pnas.1324215111
DO - 10.1073/pnas.1324215111
M3 - Article
C2 - 24453220
AN - SCOPUS:84893822094
SN - 0027-8424
VL - 111
SP - 2188
EP - 2193
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 6
ER -