Enzymatically prepared RNAi libraries

Frank Buchholz; Ralf Kittler; Mikolaj Slabicki; Mirko Theis

doi:10.1038/nmeth912

Enzymatically prepared RNAi libraries

Frank Buchholz, Ralf Kittler, Mikolaj Slabicki, Mirko Theis

Research output: Contribution to journal › Review article › peer-review

62 Scopus citations

Abstract

Large-scale RNA interference (RNAi) screens in mammalian cells have mainly used synthetic small interfering RNA (siRNA) or short hairpin RNA (shRNA) libraries. The RNAi triggers for both of these approaches were designed with algorithm-based predictions to identify single sequences for mRNA knockdown. Alternatives to these approaches have recently been developed using enzymatic methods. Here we describe the concepts of enzymatically prepared shRNA and siRNA libraries, and discuss their strengths and limitations.

Original language	English (US)
Pages (from-to)	696-700
Number of pages	5
Journal	Nature methods
Volume	3
Issue number	9
DOIs	https://doi.org/10.1038/nmeth912
State	Published - Sep 2006

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth912

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title = "Enzymatically prepared RNAi libraries",

abstract = "Large-scale RNA interference (RNAi) screens in mammalian cells have mainly used synthetic small interfering RNA (siRNA) or short hairpin RNA (shRNA) libraries. The RNAi triggers for both of these approaches were designed with algorithm-based predictions to identify single sequences for mRNA knockdown. Alternatives to these approaches have recently been developed using enzymatic methods. Here we describe the concepts of enzymatically prepared shRNA and siRNA libraries, and discuss their strengths and limitations.",

author = "Frank Buchholz and Ralf Kittler and Mikolaj Slabicki and Mirko Theis",

note = "Funding Information: We thank B. Habermann, V. Surendranath and E. Krausz for discussions. This work was supported by the EU grants “FunGenES” (LSHG-CT-2003-503494), “Mitocheck” (LSHG-CT-2004-503464), and by the Nationales Genomforschungsnetz 2 grant SMP-RNAi (01GR0402).",

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AU - Kittler, Ralf

AU - Slabicki, Mikolaj

AU - Theis, Mirko

N1 - Funding Information: We thank B. Habermann, V. Surendranath and E. Krausz for discussions. This work was supported by the EU grants “FunGenES” (LSHG-CT-2003-503494), “Mitocheck” (LSHG-CT-2004-503464), and by the Nationales Genomforschungsnetz 2 grant SMP-RNAi (01GR0402).

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N2 - Large-scale RNA interference (RNAi) screens in mammalian cells have mainly used synthetic small interfering RNA (siRNA) or short hairpin RNA (shRNA) libraries. The RNAi triggers for both of these approaches were designed with algorithm-based predictions to identify single sequences for mRNA knockdown. Alternatives to these approaches have recently been developed using enzymatic methods. Here we describe the concepts of enzymatically prepared shRNA and siRNA libraries, and discuss their strengths and limitations.

AB - Large-scale RNA interference (RNAi) screens in mammalian cells have mainly used synthetic small interfering RNA (siRNA) or short hairpin RNA (shRNA) libraries. The RNAi triggers for both of these approaches were designed with algorithm-based predictions to identify single sequences for mRNA knockdown. Alternatives to these approaches have recently been developed using enzymatic methods. Here we describe the concepts of enzymatically prepared shRNA and siRNA libraries, and discuss their strengths and limitations.

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Enzymatically prepared RNAi libraries

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