Endothelin is a potent secretagogue for atrial natriuretic peptide in cultured rat atrial myocytes

Yuka Fukuda, Yukio Hirata, Hiroki Yoshimi, Takatsugu Kojima, Yohnosuke Kobayashi, Masashi Yanagisawa, Tomoh Masaki

Research output: Contribution to journalArticlepeer-review

262 Scopus citations


Using cultured neonatal rat atrial cardiocytes, we have studied the effect of synthetic porcine endothelin (pET), a novel potent vasoconstrictor isolated from endothelial cells, on the release of immunoreactive (IR) rat atrial natriuretic peptide (rANP). pET stimulated IR-rANP secretion in a dose-dependent manner (10-10-10-7M) with an approximate half-maximally stimulatory dose of 2×10-10M. The pET-induced IR-rANP secretion was attenuated by Ca2+-channel blocker nicardipine, but no further stimulation was induced when combined with a Ca2+-channel agonist BAY-K 8644. pET in combination with tetradecanoyl-phorbol-acetate resulted in a synergistic effect on IR-rANP secretion. These data suggest that ET may play as an endogenous secretagogue for rANP by modulating Ca2+ influx through the voltage-dependent Ca2+-channels in atrial cardiocytes.

Original languageEnglish (US)
Pages (from-to)167-172
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Aug 30 1988

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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