TY - JOUR
T1 - Endothelin is a potent secretagogue for atrial natriuretic peptide in cultured rat atrial myocytes
AU - Fukuda, Yuka
AU - Hirata, Yukio
AU - Yoshimi, Hiroki
AU - Kojima, Takatsugu
AU - Kobayashi, Yohnosuke
AU - Yanagisawa, Masashi
AU - Masaki, Tomoh
N1 - Funding Information:
We thank Drs. S. Takata and Y. Takagi for their cooperation. This study was supported in part by Research Grants from the Ministry of Health and Welfare (62A-I, 63C-I), and the Ministry of Education, Science and Culture (62570530), Japan, and by a fund from the Morinaga Hoshikai.
PY - 1988/8/30
Y1 - 1988/8/30
N2 - Using cultured neonatal rat atrial cardiocytes, we have studied the effect of synthetic porcine endothelin (pET), a novel potent vasoconstrictor isolated from endothelial cells, on the release of immunoreactive (IR) rat atrial natriuretic peptide (rANP). pET stimulated IR-rANP secretion in a dose-dependent manner (10-10-10-7M) with an approximate half-maximally stimulatory dose of 2×10-10M. The pET-induced IR-rANP secretion was attenuated by Ca2+-channel blocker nicardipine, but no further stimulation was induced when combined with a Ca2+-channel agonist BAY-K 8644. pET in combination with tetradecanoyl-phorbol-acetate resulted in a synergistic effect on IR-rANP secretion. These data suggest that ET may play as an endogenous secretagogue for rANP by modulating Ca2+ influx through the voltage-dependent Ca2+-channels in atrial cardiocytes.
AB - Using cultured neonatal rat atrial cardiocytes, we have studied the effect of synthetic porcine endothelin (pET), a novel potent vasoconstrictor isolated from endothelial cells, on the release of immunoreactive (IR) rat atrial natriuretic peptide (rANP). pET stimulated IR-rANP secretion in a dose-dependent manner (10-10-10-7M) with an approximate half-maximally stimulatory dose of 2×10-10M. The pET-induced IR-rANP secretion was attenuated by Ca2+-channel blocker nicardipine, but no further stimulation was induced when combined with a Ca2+-channel agonist BAY-K 8644. pET in combination with tetradecanoyl-phorbol-acetate resulted in a synergistic effect on IR-rANP secretion. These data suggest that ET may play as an endogenous secretagogue for rANP by modulating Ca2+ influx through the voltage-dependent Ca2+-channels in atrial cardiocytes.
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U2 - 10.1016/S0006-291X(88)81064-7
DO - 10.1016/S0006-291X(88)81064-7
M3 - Article
C2 - 2458103
AN - SCOPUS:0023682779
SN - 0006-291X
VL - 155
SP - 167
EP - 172
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -