Elektrostaticheskiǐ metod opredeleniia aktivnosti fosfolipazy A.

V. V. Chernyǐ; V. M. Mirskiǐ; V. S. Sokolov; V. S. Markin

Elektrostaticheskiǐ metod opredeleniia aktivnosti fosfolipazy A.

Translated title of the contribution: Electrostatic method of determination of phospholipase A activity

V. V. Chernyǐ, V. M. Mirskiǐ, V. S. Sokolov, V. S. Markin

Neurology & Neurotherapeutics

Research output: Contribution to journal › Article › peer-review

Abstract

An electrostatic assay of phospholipase A activity has been developed. This assay is based on the monitoring of changes in the boundary potential of the planar lipid bilayer. The initial rate of the potential changes induced by phospholipase hydrolysis is proportional to the enzyme activity. The sensitivity of the method is up to 0.0002 Units/ml; the effect of impurities is ignorable. The method allows the use of natural phospholipase substrates and is rapid, i.e., the phospholipase activity assay takes approximately 5 minutes.

Translated title of the contribution	Electrostatic method of determination of phospholipase A activity
Original language	Russian
Pages (from-to)	445-450
Number of pages	6
Journal	Biokhimiya
Volume	55
Issue number	3
State	Published - Mar 1990

ASJC Scopus subject areas

General Chemistry

Cite this

@article{e562b892c2d84c8ca98f1b5d20fc366d,

title = "Elektrostaticheskiǐ metod opredeleniia aktivnosti fosfolipazy A.",

abstract = "An electrostatic assay of phospholipase A activity has been developed. This assay is based on the monitoring of changes in the boundary potential of the planar lipid bilayer. The initial rate of the potential changes induced by phospholipase hydrolysis is proportional to the enzyme activity. The sensitivity of the method is up to 0.0002 Units/ml; the effect of impurities is ignorable. The method allows the use of natural phospholipase substrates and is rapid, i.e., the phospholipase activity assay takes approximately 5 minutes.",

author = "Chernyǐ, {V. V.} and Mirskiǐ, {V. M.} and Sokolov, {V. S.} and Markin, {V. S.}",

year = "1990",

month = mar,

language = "Russian",

volume = "55",

pages = "445--450",

journal = "Biokhimiya",

issn = "0320-9725",

publisher = "Izdatel'stvo Nauka",

number = "3",

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TY - JOUR

T1 - Elektrostaticheskiǐ metod opredeleniia aktivnosti fosfolipazy A.

AU - Chernyǐ, V. V.

AU - Mirskiǐ, V. M.

AU - Sokolov, V. S.

AU - Markin, V. S.

PY - 1990/3

Y1 - 1990/3

N2 - An electrostatic assay of phospholipase A activity has been developed. This assay is based on the monitoring of changes in the boundary potential of the planar lipid bilayer. The initial rate of the potential changes induced by phospholipase hydrolysis is proportional to the enzyme activity. The sensitivity of the method is up to 0.0002 Units/ml; the effect of impurities is ignorable. The method allows the use of natural phospholipase substrates and is rapid, i.e., the phospholipase activity assay takes approximately 5 minutes.

AB - An electrostatic assay of phospholipase A activity has been developed. This assay is based on the monitoring of changes in the boundary potential of the planar lipid bilayer. The initial rate of the potential changes induced by phospholipase hydrolysis is proportional to the enzyme activity. The sensitivity of the method is up to 0.0002 Units/ml; the effect of impurities is ignorable. The method allows the use of natural phospholipase substrates and is rapid, i.e., the phospholipase activity assay takes approximately 5 minutes.

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UR - http://www.scopus.com/inward/citedby.url?scp=0025395002&partnerID=8YFLogxK

M3 - Article

C2 - 2354214

AN - SCOPUS:0025395002

SN - 0320-9725

VL - 55

SP - 445

EP - 450

JO - Biokhimiya

JF - Biokhimiya

IS - 3

ER -

Elektrostaticheskiǐ metod opredeleniia aktivnosti fosfolipazy A.

Abstract

ASJC Scopus subject areas

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