@article{3fdaf0f612ac4bfa824e0d52c4ea5e98,
title = "Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor",
abstract = "Here we describe TRACE (T7 polymerase-driven continuous editing), a method that enables continuous, targeted mutagenesis in human cells using a cytidine deaminase fused to T7 RNA polymerase. TRACE induces high rates of mutagenesis over multiple cell generations in genes under the control of a T7 promoter integrated in the genome. We used TRACE in a MEK1 inhibitor-resistance screen, and identified functionally correlated mutations.",
author = "Haiqi Chen and Sophia Liu and Samuel Padula and Daniel Lesman and Kettner Griswold and Allen Lin and Tongtong Zhao and Marshall, {Jamie L.} and Fei Chen",
note = "Funding Information: We thank Y. Jiang for help with generating figures, and J. Strecker for purified TN5 transposase. F.C. acknowledges support from Eric and Wendy Schmidt as funders of the Schmidt Fellows Program at the Broad Institute. This work was supported by the NIH Director{\textquoteright}s Early Independence Award (DP5-OD024583) to F.C. H.C. is supported by The Lalor Foundation. S.L. is supported by a Molecular Biophysics Training Grant (NIH/ National Institute of General Medical Sciences T32 GM008313) and the National Science Foundation (NSF) Graduate Research Fellowship Program. K.G. is supported under Graduate Fellowships from the Fannie and John Hertz Foundation, and the Charles Stark Draper Laboratory. A.L. is supported by a Paul and Daisy Soros Fellowship for New Americans and the NSF Graduate Research Fellowship Program. Publisher Copyright: {\textcopyright} 2019, The Author(s), under exclusive licence to Springer Nature America, Inc.",
year = "2020",
month = feb,
day = "1",
doi = "10.1038/s41587-019-0331-8",
language = "English (US)",
volume = "38",
pages = "165--168",
journal = "Bio/Technology",
issn = "0733-222X",
publisher = "Nature Publishing Group",
number = "2",
}