TY - GEN
T1 - Early assessment of nonalcoholic fatty liver disease using multiparametric ultrasound imaging
AU - Basavarajappa, Lokesh
AU - Reddy, Shreya
AU - Tai, Haowei
AU - Song, Jane
AU - Rijal, Girdhari
AU - Parker, Kevin J.
AU - Hoyt, Kenneth
N1 - Publisher Copyright:
© 2020 IEEE.
PY - 2020/9/7
Y1 - 2020/9/7
N2 - Nonalcoholic fatty liver disease (NAFLD) is the most common cause of liver disease. Patients can be characterized on the NAFLD spectrum by the progressive presence of hepatic steatosis, inflammation, hepatocyte ballooning, and then fibrosis. Currently, liver biopsy remains the only test available to detect the histologic features of NAFLD, but it is limited by its invasiveness, proneness to sampling error, and potential complications. There is an ongoing clinical need for the capability to noninvasively detect, accurately stage, and reliably monitor NAFLD. The objective of this research project was to introduce a multiparametric ultrasound (mpUS) imaging approach and evaluate its use for assessing NALFD, thereby introducing a surrogate biomarker comparable to liver biopsy. The novel method combines contrast-enhanced ultrasound (CEUS), shear wave elastography (SWE), and H-scan ultrasound (US) imaging. This approach integrates information related to liver tissue perfusion, viscoelasticity, and US scatterer size. Using Sprague-Dawley rats that were fed either control or a methionine and choline deficient (MCD) diet (N=6 per group), mpUS imaging was performed at week 0 (baseline), 2 and 6. Thereafter, animals were euthanized and livers excised for histological processing and analysis. In vivo mpUS results from control and diet fed animals revealed that all parametric measures were statistically different at 6 weeks. Histological results revealed the presence of steatosis and mild fibrosis in animals fed the MCD diet. Overall, mpUS imaging was shown to be a promising approach for the early assessment of NAFLD.
AB - Nonalcoholic fatty liver disease (NAFLD) is the most common cause of liver disease. Patients can be characterized on the NAFLD spectrum by the progressive presence of hepatic steatosis, inflammation, hepatocyte ballooning, and then fibrosis. Currently, liver biopsy remains the only test available to detect the histologic features of NAFLD, but it is limited by its invasiveness, proneness to sampling error, and potential complications. There is an ongoing clinical need for the capability to noninvasively detect, accurately stage, and reliably monitor NAFLD. The objective of this research project was to introduce a multiparametric ultrasound (mpUS) imaging approach and evaluate its use for assessing NALFD, thereby introducing a surrogate biomarker comparable to liver biopsy. The novel method combines contrast-enhanced ultrasound (CEUS), shear wave elastography (SWE), and H-scan ultrasound (US) imaging. This approach integrates information related to liver tissue perfusion, viscoelasticity, and US scatterer size. Using Sprague-Dawley rats that were fed either control or a methionine and choline deficient (MCD) diet (N=6 per group), mpUS imaging was performed at week 0 (baseline), 2 and 6. Thereafter, animals were euthanized and livers excised for histological processing and analysis. In vivo mpUS results from control and diet fed animals revealed that all parametric measures were statistically different at 6 weeks. Histological results revealed the presence of steatosis and mild fibrosis in animals fed the MCD diet. Overall, mpUS imaging was shown to be a promising approach for the early assessment of NAFLD.
KW - Contrast-enhanced ultrasound
KW - Fatty liver disease
KW - H-scan ultrasound
KW - Shear wave elastography
KW - Tissue characterization
UR - http://www.scopus.com/inward/record.url?scp=85097889456&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85097889456&partnerID=8YFLogxK
U2 - 10.1109/IUS46767.2020.9251437
DO - 10.1109/IUS46767.2020.9251437
M3 - Conference contribution
AN - SCOPUS:85097889456
T3 - IEEE International Ultrasonics Symposium, IUS
BT - IUS 2020 - International Ultrasonics Symposium, Proceedings
PB - IEEE Computer Society
T2 - 2020 IEEE International Ultrasonics Symposium, IUS 2020
Y2 - 7 September 2020 through 11 September 2020
ER -