Dynamics of the PI3K-like protein kinase members ATM and DNA-PKcs at DNA double strand breaks

Anthony J. Davis; Sairei So; David J. Chen

doi:10.4161/cc.9.13.12148

Dynamics of the PI3K-like protein kinase members ATM and DNA-PKcs at DNA double strand breaks

Anthony J. Davis, Sairei So, David J. Chen

Research output: Contribution to journal › Review article › peer-review

31 Scopus citations

Abstract

The protein kinases ATM and DNA-PKcs play critical roles in the cellular response to DNA double strand breaks (DSBs). ATM and DNA-PKcs are activated in response to DSBs and play several important roles in propagation of the damage signal and for the repair of DNA damage. Recent work from several groups, including ours, has focused on studying the dynamics of each of these proteins at DSBs and the requirements and factors which play a role(s) in this process. The use of live cell imaging of fluorescently-tagged ATM and DNA-PKcs has allowed us to study the real-time response of these proteins to laser-generated DNA damage in vivo. Here, we will extensively discuss the behavior of the ATM and DNA-PKcs proteins at DSB sites.

Original language	English (US)
Pages (from-to)	2529-2536
Number of pages	8
Journal	Cell Cycle
Volume	9
Issue number	13
DOIs	https://doi.org/10.4161/cc.9.13.12148
State	Published - Jul 1 2010

Keywords

ATM
Autophosphorylation
DNA double strand breaks
DNA-PKcs
Live cell imaging

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

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10.4161/cc.9.13.12148

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title = "Dynamics of the PI3K-like protein kinase members ATM and DNA-PKcs at DNA double strand breaks",

abstract = "The protein kinases ATM and DNA-PKcs play critical roles in the cellular response to DNA double strand breaks (DSBs). ATM and DNA-PKcs are activated in response to DSBs and play several important roles in propagation of the damage signal and for the repair of DNA damage. Recent work from several groups, including ours, has focused on studying the dynamics of each of these proteins at DSBs and the requirements and factors which play a role(s) in this process. The use of live cell imaging of fluorescently-tagged ATM and DNA-PKcs has allowed us to study the real-time response of these proteins to laser-generated DNA damage in vivo. Here, we will extensively discuss the behavior of the ATM and DNA-PKcs proteins at DSB sites.",

keywords = "ATM, Autophosphorylation, DNA double strand breaks, DNA-PKcs, Live cell imaging",

author = "Davis, {Anthony J.} and Sairei So and Chen, {David J.}",

note = "Funding Information: We thank Eric Weterings and Kazi Fattah for their critical reading of the manuscript. This work was supported by grants from the National Institutes of Health (CA50519, CA134991 and PO1-CA92584).",

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doi = "10.4161/cc.9.13.12148",

language = "English (US)",

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AU - So, Sairei

AU - Chen, David J.

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N2 - The protein kinases ATM and DNA-PKcs play critical roles in the cellular response to DNA double strand breaks (DSBs). ATM and DNA-PKcs are activated in response to DSBs and play several important roles in propagation of the damage signal and for the repair of DNA damage. Recent work from several groups, including ours, has focused on studying the dynamics of each of these proteins at DSBs and the requirements and factors which play a role(s) in this process. The use of live cell imaging of fluorescently-tagged ATM and DNA-PKcs has allowed us to study the real-time response of these proteins to laser-generated DNA damage in vivo. Here, we will extensively discuss the behavior of the ATM and DNA-PKcs proteins at DSB sites.

AB - The protein kinases ATM and DNA-PKcs play critical roles in the cellular response to DNA double strand breaks (DSBs). ATM and DNA-PKcs are activated in response to DSBs and play several important roles in propagation of the damage signal and for the repair of DNA damage. Recent work from several groups, including ours, has focused on studying the dynamics of each of these proteins at DSBs and the requirements and factors which play a role(s) in this process. The use of live cell imaging of fluorescently-tagged ATM and DNA-PKcs has allowed us to study the real-time response of these proteins to laser-generated DNA damage in vivo. Here, we will extensively discuss the behavior of the ATM and DNA-PKcs proteins at DSB sites.

KW - ATM

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KW - DNA double strand breaks

KW - DNA-PKcs

KW - Live cell imaging

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Dynamics of the PI3K-like protein kinase members ATM and DNA-PKcs at DNA double strand breaks

Abstract

Keywords

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