@article{c1146a0775ac4e2d828853108b14a6c5,
title = "Dynamic structural order of a low-complexity domain facilitates assembly of intermediate filaments",
abstract = "The coiled-coil domains of intermediate filament (IF) proteins are flanked by regions of low sequence complexity. Whereas IF coiledcoil domains assume dimeric and tetrameric conformations on their own, maturation of eight tetramers into cylindrical IFs is dependent on either {"}head{"} or {"}tail{"} domains of low sequence complexity. Here we confirm that the tail domain required for assembly of Drosophila Tm1-I/C IFs functions by forming labile cross-β interactions. These interactions are seen in polymers made from the tail domain alone, as well as in assembled IFs formed by the intact Tm1-I/C protein. The ability to visualize such interactions in situ within the context of a discrete cellular assembly lends support to the concept that equivalent interactions may be used in organizing other dynamic aspects of cell morphology.",
keywords = "Cross-beta polymerization, Intermediate filaments, Low-complexity proteins, Solid-state NMR",
author = "Sysoev, {Vasiliy O.} and Masato Kato and Lillian Sutherland and Rong Hu and McKnight, {Steven L.} and Murray, {Dylan T.}",
note = "Funding Information: ACKNOWLEDGMENTS. We thank Robert Tycko for thoughtful discussions regarding all aspects of the research described herein. We also thank Ivan Hung for assistance with the NMR spectrometers at the Tallahassee, Florida, facility, and staff of the Electron Microscopy Core Facility at UT Southwestern. S.L.M. was supported by National Institute of General Medical Sciences Grant 5R35GM13130358, as well as by unrestricted funding provided by an anonymous donor. D.T.M. was supported by faculty startup funding provided by the University of California, Davis. A portion of this work was performed at the National High-Magnetic Field Laboratory, which is supported by NSF Cooperative Agreement DMR-1644779 and the State of Florida. Publisher Copyright: {\textcopyright} 2020 National Academy of Sciences. All rights reserved.",
year = "2020",
month = sep,
day = "22",
doi = "10.1073/pnas.2010000117",
language = "English (US)",
volume = "117",
pages = "23510--23518",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "38",
}