@article{392eefdb4f3e4aaa9a8a18e92b5ec50a,
title = "Dual-Color Single-Cell Imaging of the Suprachiasmatic Nucleus Reveals a Circadian Role in Network Synchrony",
abstract = "Shan et al. generate a Cre-inducible mouse reporter with green and red luciferases fused to PER2, enabling cell-specific circadian measurements with a dual-color imaging device. Using genetically directed bioluminescence from AVP or VIP neurons in the SCN circadian pacemaker, they unravel distinct functions of the circadian clock in these neuronal subtypes.",
keywords = "Per2 gene, SCN, bioluminescence imaging, circadian rhythms, luciferase, neuronal coupling, neuronal network, suprachiasmatic nucleus",
author = "Yongli Shan and Abel, {John H.} and Yan Li and Mariko Izumo and Cox, {Kimberly H.} and Byeongha Jeong and Yoo, {Seung Hee} and Olson, {David P.} and Doyle, {Francis J.} and Takahashi, {Joseph S.}",
note = "Funding Information: Research was supported by the Howard Hughes Medical Institute (to J.S.T.), NIH/NINDS grant R01 NS106657 (to J.S.T.), Welch Foundation AU-1971-20180324 and R01 GM114424 (to S.-H.Y.), and NIH awards T32-HLO9701 and F32-AG064886 (to J.H.A.). We thank Lisa Thomas, Delali Bassowou, and Chryshanthi Joseph for assistance with animal care and maintenance and Dr. Shin Yamazaki and Dr. Carla B. Green for helpful discussions. The constructs of click-beetle luciferases were donated by Dr. David Piwnica-Worms at MD Anderson Cancer Center. Blastocyst injection was performed at the transgenic core of UT Southwestern directed by Dr. Robert E. Hammer; the JM8.F6 embryonic stem cell (ESC) line was also from the transgenic core. The Puro.Cre empty vector was a gift from Tyler Jacks (Addgene plasmid 17408). The pGEX-2T-mLIF plasmid was a gift from Dr. Janet Rossant at University of Toronto. J.S.T. is an Investigator in the Howard Hughes Medical Institute. Funding Information: Research was supported by the Howard Hughes Medical Institute (to J.S.T.), NIH/NINDS grant R01 NS106657 (to J.S.T.), Welch Foundation AU-1971-20180324 and R01 GM114424 (to S.-H.Y.), and NIH awards T32-HLO9701 and F32-AG064886 (to J.H.A.). We thank Lisa Thomas, Delali Bassowou, and Chryshanthi Joseph for assistance with animal care and maintenance and Dr. Shin Yamazaki and Dr. Carla B. Green for helpful discussions. The constructs of click-beetle luciferases were donated by Dr. David Piwnica-Worms at MD Anderson Cancer Center. Blastocyst injection was performed at the transgenic core of UT Southwestern directed by Dr. Robert E. Hammer; the JM8.F6 embryonic stem cell (ESC) line was also from the transgenic core. The Puro.Cre empty vector was a gift from Tyler Jacks (Addgene plasmid 17408). The pGEX-2T-mLIF plasmid was a gift from Dr. Janet Rossant at University of Toronto. J.S.T. is an Investigator in the Howard Hughes Medical Institute. Experimental design, Y.S. and J.S.T.; targeting vector, Y.S. and S.-H.Y.; ESC targeting and mouse line development, Y.S. and Y.L.; AVP-iCre mouse, D.P.O.; dual-color imaging device development, Y.S.; performing experiments and data acquisition, Y.S. Y.L. and M.I.; data analysis, interpretation, and generation of figures, Y.S. J.H.A. B.J. and K.H.C.; computational modeling, coding, and automation, J.H.A.; writing, reviewing, and editing the manuscript, J.S.T. Y.S. J.H.A. and K.H.C.; scientific direction and funding, J.S.T. and F.J.D. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = oct,
day = "14",
doi = "10.1016/j.neuron.2020.07.012",
language = "English (US)",
volume = "108",
pages = "164--179.e7",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "1",
}