TY - JOUR
T1 - Draxin from neocortical neurons controls the guidance of thalamocortical projections into the neocortex
AU - Shinmyo, Yohei
AU - Riyadh, M. Asrafuzzaman
AU - Ahmed, Giasuddin
AU - Naser, Iftekhar Bin
AU - Hossain, Mahmud
AU - Takebayashi, Hirohide
AU - Kawasaki, Hiroshi
AU - Ohta, Kunimasa
AU - Tanaka, Hideaki
N1 - Funding Information:
We thank M. Tessier-Lavigne for the Dcc, Unc5a, Unc5b and Neo1 mice; K. Yamakawa for the Dscam mice; T. Iwasato and S. Itohara for the Emx1Cre mice; Dr M. Okabe for the EGFP mice; T. Tojima and H. Kamiguchi for the L1-Fc plasmids; and S. Garel for the Lhx6 and Ebf1 plasmids. We also thank R. Koyama, A. Kubota, K. Yamada, A. Hamashima and M. Kumamaru for technical assistance; K. Watanabe and K. Shimamura for helpful discussions; and the Developmental Studies Hybridoma Bank for antibodies. This work was supported by JSPS Grants-in-Aid for Scientific Research (S) 23220010 (H. Tanaka) and Young Scientists (A) 23680036 (Y.S.); MEXT Grant-in-Aid for Scientific Research on Innovative Areas 26110715 and 23123517 (Y.S.); grants from the Takeda Science Foundation (Y.S.) and the Brain Science Foundation (Y.S.). This work is dedicated to Dr Hideaki Tanaka.
PY - 2015
Y1 - 2015
N2 - The thalamocortical tract carries sensory information to the neocortex. It has long been recognized that the neocortical pioneer axons of subplate neurons are essential for thalamocortical development. Herein we report that an axon guidance cue, draxin, is expressed in early-born neocortical neurons, including subplate neurons, and is necessary for thalamocortical development. In draxin-/- mice, thalamocortical axons do not enter the neocortex. This phenotype is sufficiently rescued by the transgenic expression of draxin in neocortical neurons. Genetic interaction data suggest that draxin acts through Deleted in colorectal cancer (DCC) and Neogenin (Neo1), to regulate thalamocortical projections in vivo. Draxin promotes the outgrowth of thalamic axons in vitro and this effect is abolished in thalamic neurons from Dcc and Neo1 double mutants. These results suggest that draxin from neocortical neurons controls thalamocortical projections into the neocortex, and that this effect is mediated through the DCC and Neo1 receptors.
AB - The thalamocortical tract carries sensory information to the neocortex. It has long been recognized that the neocortical pioneer axons of subplate neurons are essential for thalamocortical development. Herein we report that an axon guidance cue, draxin, is expressed in early-born neocortical neurons, including subplate neurons, and is necessary for thalamocortical development. In draxin-/- mice, thalamocortical axons do not enter the neocortex. This phenotype is sufficiently rescued by the transgenic expression of draxin in neocortical neurons. Genetic interaction data suggest that draxin acts through Deleted in colorectal cancer (DCC) and Neogenin (Neo1), to regulate thalamocortical projections in vivo. Draxin promotes the outgrowth of thalamic axons in vitro and this effect is abolished in thalamic neurons from Dcc and Neo1 double mutants. These results suggest that draxin from neocortical neurons controls thalamocortical projections into the neocortex, and that this effect is mediated through the DCC and Neo1 receptors.
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U2 - 10.1038/ncomms10232
DO - 10.1038/ncomms10232
M3 - Article
C2 - 26659141
AN - SCOPUS:84986879599
SN - 2041-1723
VL - 6
JO - Nature communications
JF - Nature communications
M1 - 10232
ER -