@article{d1b5b8464ff64bfb93f326f528232bd0,
title = "Dosing a synbiotic of human milk oligosaccharides and B. infantis leads to reversible engraftment in healthy adult microbiomes without antibiotics",
abstract = "Predictable and sustainable engraftment of live biotherapeutic products into the human gut microbiome is being explored as a promising way to modulate the human gut microbiome. We utilize a synbiotic approach pairing the infant gut microbe Bifidobacterium longum subspecies infantis (B. infantis) and human milk oligosaccharides (HMO). B. infantis, which is typically absent in adults, engrafts into healthy adult microbiomes in an HMO-dependent manner at a relative abundance of up to 25% of the bacterial population without antibiotic pretreatment or adverse effects. Corresponding changes in metabolites are detected. Germ-free mice transplanted with dysbiotic human microbiomes also successfully engraft with B. infantis in an HMO-dependent manner, and the synbiotic augments butyrate levels both in this in vivo model and in in vitro cocultures of the synbiotic with specific Firmicutes species. Finally, the synbiotic inhibits the growth of enteropathogens in vitro. Our findings point to a potential safe mechanism for ameliorating dysbioses characteristic of numerous human diseases.",
keywords = "B. infantis, Bifidobacterium, gut engraftment, gut microbiome, gut microbiota, HMO, human milk oligosaccharides, LBP, live biotherapeutic product, microbiome modulation",
author = "Button, {Julie E.} and Autran, {Chloe A.} and Reens, {Abigail L.} and Cosetta, {Casey M.} and Steven Smriga and Megan Ericson and Pierce, {Jessica V.} and Cook, {David N.} and Lee, {Martin L.} and Sun, {Adam K.} and Alousi, {Amin M.} and Koh, {Andrew Y.} and Rechtman, {David J.} and Jenq, {Robert R.} and McKenzie, {Gregory J.}",
note = "Funding Information: We would like to thank Biranchi Patra, Russell Saur, Rook Zheng, Kim Long, Nicola Gerty, Shao-Hung Fred Tsen, Huiyu Xia, KimThu Tran, Tin Huynh, Thanhvan Nguyen, Anita Wong, Jorge Ramirez, Gildardo Inzunza, Yesenia Martinez, Vera Chan, and Katie Van Heirseele for their support of and contributions to this study. This work was funded by Prolacta Bioscience. Conceptualization, J.E.B. C.A.A. D.N.C. and G.J.M.; methodology, J.E.B. A.L.R. M.E. J.V.P. and G.J.M.; formal analysis, M.L.L.; investigation, J.E.B. A.L.R. C.M.C. M.E. and J.V.P.; resources, A.K.S. and R.R.J.; data curation, A.L.R. C.M.C. and S.S.; writing – original draft, J.E.B. A.L.R. C.M.C. and S.S.; writing – review & editing, J.E.B. C.A.A. A.L.R. C.M.C. S.S. D.N.C. M.L.L. A.K.S. A.M.A. A.Y.K. D.J.R. R.R.J. and G.J.M.; visualization, J.E.B. A.L.R. C.M.C. and S.S.; supervision, J.E.B. M.E. M.L.L. A.K.S. A.M.A. A.Y.K. D.J.R. R.R.J. and G.J.M.; funding acquisition, D.N.C. and G.J.M. Prolacta Bioscience employees are also shareholders in the company. D.N.C. served as a consultant to Prolacta Bioscience. A.M.A. A.Y.K. and R.R.J. are members of Prolacta Bioscience's clinical advisory board. U.S. Patent No. 8,927,027, International Application Pub. No. WO 2021/061991, and their corresponding family member patents and applications are related to this work. Funding Information: We would like to thank Biranchi Patra, Russell Saur, Rook Zheng, Kim Long, Nicola Gerty, Shao-Hung Fred Tsen, Huiyu Xia, KimThu Tran, Tin Huynh, Thanhvan Nguyen, Anita Wong, Jorge Ramirez, Gildardo Inzunza, Yesenia Martinez, Vera Chan, and Katie Van Heirseele for their support of and contributions to this study. This work was funded by Prolacta Bioscience . Publisher Copyright: {\textcopyright} 2022 Elsevier Inc.",
year = "2022",
month = may,
day = "11",
doi = "10.1016/j.chom.2022.04.001",
language = "English (US)",
volume = "30",
pages = "712--725.e7",
journal = "Cell Host and Microbe",
issn = "1931-3128",
publisher = "Cell Press",
number = "5",
}