@inproceedings{ca29ee14dc964707b4a4311e49dc7771,
title = "DNA sequencing using four-color capillary array electrophoresis and energy-transfer primers",
abstract = "A practical capillary array electrophoresis sequencer has been constructed and tested for large scale DNA sequencing. This system employs a scanning confocal optical system to detect DNA fragments electrophoresed on a capillary array. By using the recently developed energy transfer fluorescent primers, sensitive four-color detection is facilitated while exciting with just a single laser wavelength (488 nm) from an argon ion laser. Software for facile reduction of the images to four-color trace files and for automated base-calling have also been developed. This system can detect up to 25 capillaries at a time and has a raw sequencing rate of approximately 6 kilobases/hour. Applications to mitochondrial D-loop DNA sequencing are presented as a demonstration.",
author = "Indu Kheterpal and Jingyue Ju and Arun Radhakrishnan and Brandt, {Gabriel S.} and Ginther, {Charles L.} and Clark, {Steven M.} and Scherer, {James R.} and Sensabaugh, {George F.} and Mathies, {Richard A.}",
year = "1996",
language = "English (US)",
isbn = "0819420549",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
pages = "204--213",
editor = "Cohn, {Gerald E.} and Soper, {Steven A.} and C.H.W. Chen",
booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",
note = "Ultrasensitive Biochemical Diagnostics ; Conference date: 31-01-1996 Through 02-02-1996",
}