Abstract
The recently developed DNA microarray technology provides a powerful and efficient tool to rapidly compare the differential expression of a large number of genes. Using the DNA microarray approach, we investigated gene expression profiles in cultured human aortic endothelial cells (HAECs) in response to 24 h of laminar shear stress at 12 dyn/cm2. This relatively long-term shearing of cultured HAECs led to the modulation of the expression of a number of genes. Several genes related to inflammation and EC proliferation were downregulated, suggesting that 24-h shearing may keep ECs in a relatively noninflammatory and nonproliferative state compared with static cells. Some genes were significantly upregulated by the 24-h shear stress; these includes genes involved in EC survival and angiogenesis (Tie2 and Flk-1) and vascular remodeling (matrix metalloproteinase 1). These results provide information on the profile of gene expression in shear-adapted ECs, which is the case for the native ECs in the straight part of the aorta in vivo.
Original language | English (US) |
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Pages (from-to) | 55-63 |
Number of pages | 9 |
Journal | Physiological genomics |
Volume | 2002 |
Issue number | 7 |
State | Published - Jan 2002 |
Keywords
- Dna microarray
- Endothelial cells
- Gene expression
- Shear stress
ASJC Scopus subject areas
- Physiology
- Genetics