DLP hyperspectral imaging for surgical and clinical utility

Karel J. Zuzak, Robert P. Francis, Eleanor F. Wehner, Jack Smith, Maritoni Litorja, David W. Allen, Chad Tracy, Jeffrey A Cadeddu, Edward Livingston

Research output: Chapter in Book/Report/Conference proceedingConference contribution

20 Scopus citations


We describe a novel digital light processing, DLP hyperspectral imaging system for visualizing chemical composition of in vivo tissues during surgical procedures non-invasively and at near video rate. The novelty of the DLP hyperspectral imaging system resides in (1) its ability to conform light to rapidly sweep through a series of preprogrammed spectral illuminations as simple as a set of contiguous bandpasses to any number of complex spectra, and (2) processing the reflected spectroscopic image data using unique supervised and unsupervised chemometric methods that color encode molecular content of tissue at each image detector pixel providing an optical biopsy. Spectral illumination of tissue is accomplished utilizing a DLP® based spectral illuminator incorporating a series of bandpass spectra and measuring the reflectance image with a CCD array detector. Wavelength dependent images are post processed with a multivariate least squares analysis method using known reference spectra of oxy- and deoxy-hemoglobin. Alternatively, illuminating with complex reference spectra reduces the number of spectral images required for generating chemically relevant images color encoded for relative percentage of oxyhemoglobin are collected and displayed in real time near-video rate, (3 to 4) frames per second (fps). As a proof of principle application, a kidney of an anesthetized pig was imaged before and after renal vasculature occlusion showing the clamped kidney to be 61% of the undamped kidney percentage of oxyhemoglobin. Using the "3-Shot" spectral illumination method and gathering data at (3 to 4) fps shows a non-linear exponential de-oxygenation of hemoglobin reaching steady state within 30 seconds post occlusion.

Original languageEnglish (US)
Title of host publicationEmerging Digital Micromirror Device Based Systems and Applications
StatePublished - Jun 29 2009
EventEmerging Digital Micromirror Device Based Systems and Applications - San Jose, CA, United States
Duration: Jan 28 2009Jan 28 2009

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X


OtherEmerging Digital Micromirror Device Based Systems and Applications
Country/TerritoryUnited States
CitySan Jose, CA


  • Digital light processing (DLP)
  • Digital micromirror device (DMD)
  • Hyperspectral imaging
  • Optical biopsy
  • Oxyhemoglobin
  • Renal artery occlusion
  • System development

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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