Distribution of heat shock protein 108 mRNA during the development of the chicken brain

Dong Hoon Shin; Eunju Lee; Jaehyup Kim; Su Ryeon Bae; Yoon Hee Chung; Hyun Joon Kim; Mun Han Lee; Sa Sun Cho

doi:10.1016/j.neulet.2004.08.032

Distribution of heat shock protein 108 mRNA during the development of the chicken brain

Dong Hoon Shin, Eunju Lee, Jaehyup Kim, Su Ryeon Bae, Yoon Hee Chung, Hyun Joon Kim, Mun Han Lee, Sa Sun Cho

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The developmental expression of heat shock protein 108 (HSP108) mRNA was mapped in chicken brain using in situ hybridization and reverse transcription-polymerase chain reaction (RT-PCR). RT-PCR showed that HSP108 mRNA increased from embryonic day 5 (E5) to 13 (E13), significantly decreased from E17 to E21 and then increased again at the adult stage. In situ hybridization showed that while intense HSP108 positive (HSP108 ⁺) signals were localized in the cerebellum from E7 to E14, the intensities of these signals were significantly decreased at E17. However, at the adult stage, HSP108 expression increased in a cell type dependent manner. A decrease in HSP108 mRNA expression during differentiation was also observed in an in vitro study of brain cells treated with nerve growth factor (NGF).

Original language	English (US)
Pages (from-to)	140-145
Number of pages	6
Journal	Neuroscience letters
Volume	370
Issue number	2-3
DOIs	https://doi.org/10.1016/j.neulet.2004.08.032
State	Published - Nov 11 2004
Externally published	Yes

Keywords

Bergmann glial cell
Brain
Chicken
Heat shock protein
In situ hybridization
Nerve growth factor

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.neulet.2004.08.032

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@article{c94dedbc7477494db8be53fe2e240eda,

title = "Distribution of heat shock protein 108 mRNA during the development of the chicken brain",

abstract = "The developmental expression of heat shock protein 108 (HSP108) mRNA was mapped in chicken brain using in situ hybridization and reverse transcription-polymerase chain reaction (RT-PCR). RT-PCR showed that HSP108 mRNA increased from embryonic day 5 (E5) to 13 (E13), significantly decreased from E17 to E21 and then increased again at the adult stage. In situ hybridization showed that while intense HSP108 positive (HSP108 +) signals were localized in the cerebellum from E7 to E14, the intensities of these signals were significantly decreased at E17. However, at the adult stage, HSP108 expression increased in a cell type dependent manner. A decrease in HSP108 mRNA expression during differentiation was also observed in an in vitro study of brain cells treated with nerve growth factor (NGF).",

keywords = "Bergmann glial cell, Brain, Chicken, Heat shock protein, In situ hybridization, Nerve growth factor",

author = "Shin, {Dong Hoon} and Eunju Lee and Jaehyup Kim and Bae, {Su Ryeon} and Chung, {Yoon Hee} and Kim, {Hyun Joon} and Lee, {Mun Han} and Cho, {Sa Sun}",

note = "Funding Information: This study was supported by a Korea Research Foundation Grant (KRF 2001-042-F00005).",

year = "2004",

month = nov,

day = "11",

doi = "10.1016/j.neulet.2004.08.032",

language = "English (US)",

volume = "370",

pages = "140--145",

journal = "Neuroscience letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

number = "2-3",

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T1 - Distribution of heat shock protein 108 mRNA during the development of the chicken brain

AU - Shin, Dong Hoon

AU - Lee, Eunju

AU - Kim, Jaehyup

AU - Bae, Su Ryeon

AU - Chung, Yoon Hee

AU - Kim, Hyun Joon

AU - Lee, Mun Han

AU - Cho, Sa Sun

N1 - Funding Information: This study was supported by a Korea Research Foundation Grant (KRF 2001-042-F00005).

PY - 2004/11/11

Y1 - 2004/11/11

N2 - The developmental expression of heat shock protein 108 (HSP108) mRNA was mapped in chicken brain using in situ hybridization and reverse transcription-polymerase chain reaction (RT-PCR). RT-PCR showed that HSP108 mRNA increased from embryonic day 5 (E5) to 13 (E13), significantly decreased from E17 to E21 and then increased again at the adult stage. In situ hybridization showed that while intense HSP108 positive (HSP108 +) signals were localized in the cerebellum from E7 to E14, the intensities of these signals were significantly decreased at E17. However, at the adult stage, HSP108 expression increased in a cell type dependent manner. A decrease in HSP108 mRNA expression during differentiation was also observed in an in vitro study of brain cells treated with nerve growth factor (NGF).

AB - The developmental expression of heat shock protein 108 (HSP108) mRNA was mapped in chicken brain using in situ hybridization and reverse transcription-polymerase chain reaction (RT-PCR). RT-PCR showed that HSP108 mRNA increased from embryonic day 5 (E5) to 13 (E13), significantly decreased from E17 to E21 and then increased again at the adult stage. In situ hybridization showed that while intense HSP108 positive (HSP108 +) signals were localized in the cerebellum from E7 to E14, the intensities of these signals were significantly decreased at E17. However, at the adult stage, HSP108 expression increased in a cell type dependent manner. A decrease in HSP108 mRNA expression during differentiation was also observed in an in vitro study of brain cells treated with nerve growth factor (NGF).

KW - Bergmann glial cell

KW - Brain

KW - Chicken

KW - Heat shock protein

KW - In situ hybridization

KW - Nerve growth factor

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DO - 10.1016/j.neulet.2004.08.032

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C2 - 15488311

AN - SCOPUS:5644275372

SN - 0304-3940

VL - 370

SP - 140

EP - 145

JO - Neuroscience letters

JF - Neuroscience letters

IS - 2-3

ER -

Distribution of heat shock protein 108 mRNA during the development of the chicken brain

Abstract

Keywords

ASJC Scopus subject areas

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