TY - JOUR
T1 - Dissecting primate early post-implantation development using long-term in vitro embryo culture
AU - Niu, Yuyu
AU - Sun, Nianqin
AU - Li, Chang
AU - Lei, Ying
AU - Huang, Zhihao
AU - Wu, Jun
AU - Si, Chenyang
AU - Dai, Xi
AU - Liu, Chuanyu
AU - Wei, Jingkuan
AU - Liu, Longqi
AU - Feng, Su
AU - Kang, Yu
AU - Si, Wei
AU - Wang, Hong
AU - Zhang, E.
AU - Zhao, Lu
AU - Li, Ziwei
AU - Luo, Xi
AU - Cui, Guizhong
AU - Peng, Guangdun
AU - Belmonte, Juan Carlos Izpisúa
AU - Ji, Weizhi
AU - Tan, Tao
N1 - Funding Information:
We thank Y. Hou and S. Liu for discussion of this study and L. Xu and Y. Wang for their technical assistance. We are grateful to K. Chen, X. Lin, and X. Liu for helpful feedback on the manuscript. This work was supported by the National Key Research and Development Program (2016YFA0101401), the National Natural Science Foundation of China (81760271), Major Science and Technology Projects of Yunnan Province (2017ZF028), Key Projects of Basic Research Program in Yunnan Province (2017FA010), and Frontier Research Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory (2018GZR110105013).
Publisher Copyright:
Copyright © 2019 The Authors,
PY - 2019/11/15
Y1 - 2019/11/15
N2 - The transition from peri-implantation to gastrulation in mammals entails the specification and organization of the lineage progenitors into a body plan. Technical and ethical challenges have limited understanding of the cellular and molecular mechanisms that underlie this transition. We established a culture system that enabled the development of cynomolgus monkey embryos in vitro for up to 20 days. Cultured embryos underwent key primate developmental stages, including lineage segregation, bilaminar disc formation, amniotic and yolk sac cavitation, and primordial germ cell–like cell (PGCLC) differentiation. Single-cell RNA-sequencing analysis revealed development trajectories of primitive endoderm, trophectoderm, epiblast lineages, and PGCLCs. Analysis of single-cell chromatin accessibility identified transcription factors specifying each cell type. Our results reveal critical developmental events and complex molecular mechanisms underlying nonhuman primate embryogenesis in the early postimplantation period, with possible relevance to human development.
AB - The transition from peri-implantation to gastrulation in mammals entails the specification and organization of the lineage progenitors into a body plan. Technical and ethical challenges have limited understanding of the cellular and molecular mechanisms that underlie this transition. We established a culture system that enabled the development of cynomolgus monkey embryos in vitro for up to 20 days. Cultured embryos underwent key primate developmental stages, including lineage segregation, bilaminar disc formation, amniotic and yolk sac cavitation, and primordial germ cell–like cell (PGCLC) differentiation. Single-cell RNA-sequencing analysis revealed development trajectories of primitive endoderm, trophectoderm, epiblast lineages, and PGCLCs. Analysis of single-cell chromatin accessibility identified transcription factors specifying each cell type. Our results reveal critical developmental events and complex molecular mechanisms underlying nonhuman primate embryogenesis in the early postimplantation period, with possible relevance to human development.
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U2 - 10.1126/science.aaw5754
DO - 10.1126/science.aaw5754
M3 - Article
C2 - 31672917
AN - SCOPUS:85075094510
SN - 0036-8075
VL - 366
JO - Science
JF - Science
IS - 6467
M1 - eaaw5754
ER -