Dissecting embryonic and extraembryonic lineage crosstalk with stem cell co-culture

Yulei Wei; E. Zhang; Leqian Yu; Baiquan Ci; Masahiro Sakurai; Lei Guo; Xin Zhang; Sirui Lin; Shino Takii; Lizhong Liu; Jian Liu; Daniel A. Schmitz; Ting Su; Junmei Zhang; Qiaoyan Shen; Yi Ding; Linfeng Zhan; Hai Xi Sun; Canbin Zheng; Lin Xu; Daiji Okamura; Weizhi Ji; Tao Tan; Jun Wu

doi:10.1016/j.cell.2023.11.008

Dissecting embryonic and extraembryonic lineage crosstalk with stem cell co-culture

Yulei Wei, E. Zhang, Leqian Yu, Baiquan Ci, Masahiro Sakurai, Lei Guo, Xin Zhang, Sirui Lin, Shino Takii, Lizhong Liu, Jian Liu, Daniel A. Schmitz, Ting Su, Junmei Zhang, Qiaoyan Shen, Yi Ding, Linfeng Zhan, Hai Xi Sun, Canbin Zheng, Lin XuDaiji Okamura, Weizhi Ji, Tao Tan, Jun Wu

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Embryogenesis necessitates harmonious coordination between embryonic and extraembryonic tissues. Although stem cells of both embryonic and extraembryonic origins have been generated, they are grown in different culture conditions. In this study, utilizing a unified culture condition that activates the FGF, TGF-β, and WNT pathways, we have successfully derived embryonic stem cells (FTW-ESCs), extraembryonic endoderm stem cells (FTW-XENs), and trophoblast stem cells (FTW-TSCs) from the three foundational tissues of mouse and cynomolgus monkey (Macaca fascicularis) blastocysts. This approach facilitates the co-culture of embryonic and extraembryonic stem cells, revealing a growth inhibition effect exerted by extraembryonic endoderm cells on pluripotent cells, partially through extracellular matrix signaling. Additionally, our cross-species analysis identified both shared and unique transcription factors and pathways regulating FTW-XENs. The embryonic and extraembryonic stem cell co-culture strategy offers promising avenues for developing more faithful embryo models and devising more developmentally pertinent differentiation protocols.

Original language	English (US)
Pages (from-to)	5859-5875.e24
Journal	Cell
Volume	186
Issue number	26
DOIs	https://doi.org/10.1016/j.cell.2023.11.008
State	Published - Dec 21 2023

Keywords

ECM signaling
embryonic stem cell
extraembryonic endoderm stem cell
lineage crosstalk
monkey
mouse
trophoblast stem cell

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.cell.2023.11.008

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Wei, Y., Zhang, E., Yu, L., Ci, B., Sakurai, M., Guo, L., Zhang, X., Lin, S., Takii, S., Liu, L., Liu, J., Schmitz, D. A., Su, T., Zhang, J., Shen, Q., Ding, Y., Zhan, L., Sun, H. X., Zheng, C., ... Wu, J. (2023). Dissecting embryonic and extraembryonic lineage crosstalk with stem cell co-culture. Cell, 186(26), 5859-5875.e24. https://doi.org/10.1016/j.cell.2023.11.008

Wei, Y, Zhang, E, Yu, L, Ci, B, Sakurai, M, Guo, L, Zhang, X, Lin, S, Takii, S, Liu, L, Liu, J, Schmitz, DA, Su, T, Zhang, J, Shen, Q, Ding, Y, Zhan, L, Sun, HX, Zheng, C, Xu, L, Okamura, D, Ji, W, Tan, T & Wu, J 2023, 'Dissecting embryonic and extraembryonic lineage crosstalk with stem cell co-culture', Cell, vol. 186, no. 26, pp. 5859-5875.e24. https://doi.org/10.1016/j.cell.2023.11.008

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abstract = "Embryogenesis necessitates harmonious coordination between embryonic and extraembryonic tissues. Although stem cells of both embryonic and extraembryonic origins have been generated, they are grown in different culture conditions. In this study, utilizing a unified culture condition that activates the FGF, TGF-β, and WNT pathways, we have successfully derived embryonic stem cells (FTW-ESCs), extraembryonic endoderm stem cells (FTW-XENs), and trophoblast stem cells (FTW-TSCs) from the three foundational tissues of mouse and cynomolgus monkey (Macaca fascicularis) blastocysts. This approach facilitates the co-culture of embryonic and extraembryonic stem cells, revealing a growth inhibition effect exerted by extraembryonic endoderm cells on pluripotent cells, partially through extracellular matrix signaling. Additionally, our cross-species analysis identified both shared and unique transcription factors and pathways regulating FTW-XENs. The embryonic and extraembryonic stem cell co-culture strategy offers promising avenues for developing more faithful embryo models and devising more developmentally pertinent differentiation protocols.",

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author = "Yulei Wei and E. Zhang and Leqian Yu and Baiquan Ci and Masahiro Sakurai and Lei Guo and Xin Zhang and Sirui Lin and Shino Takii and Lizhong Liu and Jian Liu and Schmitz, {Daniel A.} and Ting Su and Junmei Zhang and Qiaoyan Shen and Yi Ding and Linfeng Zhan and Sun, {Hai Xi} and Canbin Zheng and Lin Xu and Daiji Okamura and Weizhi Ji and Tao Tan and Jun Wu",

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doi = "10.1016/j.cell.2023.11.008",

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AU - Wei, Yulei

AU - Zhang, E.

AU - Yu, Leqian

AU - Ci, Baiquan

AU - Sakurai, Masahiro

AU - Guo, Lei

AU - Zhang, Xin

AU - Lin, Sirui

AU - Takii, Shino

AU - Liu, Lizhong

AU - Liu, Jian

AU - Schmitz, Daniel A.

AU - Su, Ting

AU - Zhang, Junmei

AU - Shen, Qiaoyan

AU - Ding, Yi

AU - Zhan, Linfeng

AU - Sun, Hai Xi

AU - Zheng, Canbin

AU - Xu, Lin

AU - Okamura, Daiji

AU - Ji, Weizhi

AU - Tan, Tao

AU - Wu, Jun

PY - 2023/12/21

Y1 - 2023/12/21

N2 - Embryogenesis necessitates harmonious coordination between embryonic and extraembryonic tissues. Although stem cells of both embryonic and extraembryonic origins have been generated, they are grown in different culture conditions. In this study, utilizing a unified culture condition that activates the FGF, TGF-β, and WNT pathways, we have successfully derived embryonic stem cells (FTW-ESCs), extraembryonic endoderm stem cells (FTW-XENs), and trophoblast stem cells (FTW-TSCs) from the three foundational tissues of mouse and cynomolgus monkey (Macaca fascicularis) blastocysts. This approach facilitates the co-culture of embryonic and extraembryonic stem cells, revealing a growth inhibition effect exerted by extraembryonic endoderm cells on pluripotent cells, partially through extracellular matrix signaling. Additionally, our cross-species analysis identified both shared and unique transcription factors and pathways regulating FTW-XENs. The embryonic and extraembryonic stem cell co-culture strategy offers promising avenues for developing more faithful embryo models and devising more developmentally pertinent differentiation protocols.

AB - Embryogenesis necessitates harmonious coordination between embryonic and extraembryonic tissues. Although stem cells of both embryonic and extraembryonic origins have been generated, they are grown in different culture conditions. In this study, utilizing a unified culture condition that activates the FGF, TGF-β, and WNT pathways, we have successfully derived embryonic stem cells (FTW-ESCs), extraembryonic endoderm stem cells (FTW-XENs), and trophoblast stem cells (FTW-TSCs) from the three foundational tissues of mouse and cynomolgus monkey (Macaca fascicularis) blastocysts. This approach facilitates the co-culture of embryonic and extraembryonic stem cells, revealing a growth inhibition effect exerted by extraembryonic endoderm cells on pluripotent cells, partially through extracellular matrix signaling. Additionally, our cross-species analysis identified both shared and unique transcription factors and pathways regulating FTW-XENs. The embryonic and extraembryonic stem cell co-culture strategy offers promising avenues for developing more faithful embryo models and devising more developmentally pertinent differentiation protocols.

KW - ECM signaling

KW - embryonic stem cell

KW - extraembryonic endoderm stem cell

KW - lineage crosstalk

KW - monkey

KW - mouse

KW - trophoblast stem cell

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Dissecting embryonic and extraembryonic lineage crosstalk with stem cell co-culture

Abstract

Keywords

ASJC Scopus subject areas

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