@article{76275b3c49c34835900b60d8db2037bd,
title = "Direct measurement of conformational strain energy in protofilaments curling outward from disassembling microtubule tips",
abstract = "Disassembling microtubules can generate movement independently of motor enzymes, especially at kinetochores where they drive chromosome motility. A popular explanation is the {\textquoteleft}conformational wave{\textquoteright} model, in which protofilaments pull on the kinetochore as they curl outward from a disassembling tip. But whether protofilaments can work efficiently via this spring-like mechanism has been unclear. By modifying a previous assay to use recombinant tubulin and feedback-controlled laser trapping, we directly demonstrate the spring-like elasticity of curling protofilaments. Measuring their mechanical work output suggests they carry ~25% of the energy of GTP hydrolysis as bending strain, enabling them to drive movement with efficiency similar to conventional motors. Surprisingly, a β-tubulin mutant that dramatically slows disassembly has no effect on work output, indicating an uncoupling of disassembly speed from protofilament strain. These results show the wave mechanism can make a major contribution to kinetochore motility and establish a direct approach for measuring tubulin mechano-chemistry.",
author = "Driver, {Jonathan W.} and Geyer, {Elisabeth A.} and Bailey, {Megan E.} and Rice, {Luke M.} and Asbury, {Charles L.}",
note = "Funding Information: This work was supported by a Sackler Scholars Award in Integrative Biophysics to JWD, by a Fellow Award from the Leukemia and Lymphoma Society to JWD, by an NIH Training grant to MEB (T32CA080416), by a Packard Fellowship to CLA (2006-30521), and by grants to CLA (NIH: RO1GM079373) and LMR (NIH: RO1GM098543 and NSF CAREER: MCB1054947). EAG was supported by an NSF Graduate Research Fellowship (2014177758). Sackler Scholars Program in Integrative Biophysics Jonathan W Driver Leukemia and Lymphoma Society Jonathan W Driver National Institutes of Health T32CA080416 Megan E Bailey David and Lucile Packard2006-30521 Charles L Asbury ational Science Foundation Graduate Research Fellowship 2014177758 Elisabeth A Geyer National Institutes of Health RO1GM098543 Luke M Rice National Science Foundation Career Award MCB1054947 Luke M Rice National Institutes of Health RO1GM079373 Charles L Asbury The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. Publisher Copyright: {\textcopyright} Driver et al.",
year = "2017",
month = jun,
day = "19",
doi = "10.7554/eLife.28433",
language = "English (US)",
volume = "6",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}