Abstract
The asymmetry in the doublet of a spin coupled to hyperpolarized 13C has been used previously to measure the initial polarization of 13C. We tested the hypothesis that a single observation of the 1H NMR spectrum of hyperpolarized 13C formate monitors 13C polarization. Depending on the microwave frequency during the polarization process, in-phase or out-of-phase doublets were observed in the 1H NMR spectrum. Even in this simple two-spin system, 13C polarization was not reflected in the relative area of the JCH doublet components due to strong heteronuclear cross-relaxation. The Solomon equations were used to model the proton signal as a function of time after polarization and to estimate 13C polarization from the 1H NMR spectra.
Original language | English (US) |
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Pages (from-to) | 280-285 |
Number of pages | 6 |
Journal | Journal of Magnetic Resonance |
Volume | 189 |
Issue number | 2 |
DOIs | |
State | Published - Dec 2007 |
Keywords
- Dynamic nuclear polarization
- Formate
- Overhauser effect
- SPINOE
- Solomon equations
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Nuclear and High Energy Physics
- Condensed Matter Physics