TY - JOUR
T1 - Differential mitotic activation of endogenous c-Src, c-Yes, and Lyn in HeLa cells
AU - Kuga, Takahisa
AU - Nakayama, Yuji
AU - Hoshino, Masaki
AU - Higashiyama, Yukihiro
AU - Obata, Yuuki
AU - Matsuda, Daisuke
AU - Kasahara, Kousuke
AU - Fukumoto, Yasunori
AU - Yamaguchi, Naoto
N1 - Funding Information:
We are indebted to Dr. R.Y.C. Poon (The Hong Kong University of Science and Technology), Dr. J. Pines (University of Cambridge), Dr. D.J. Fujita (University of Calgary), and Dr. T. Yamamoto (The University of Tokyo) for valuable plasmids and to Dr. K. Igarashi (Chiba University), Dr. M.N. Fukuda (The Burnham Institute for Medical Research), and Dr. M. Tagawa (Chiba Cancer Center Research Institute) for cell lines. This work was supported in part by Grants-in-Aid for Scientific Research from the Japanese Ministry of Education, Culture, Sports, Science and Technology, and a research grant from the Takeda Science Foundation. K.K. was supported by a Research Fellowship of the Japanese Society for the Promotion of Science Research (JSPS) for Young Scientists.
PY - 2007/10/1
Y1 - 2007/10/1
N2 - Src-family tyrosine kinases (SFKs) play an important role in mitosis. Despite overlapping expression of multiple SFK members, little is known about how individual SFK members are activated in M phase. Here, we examined mitotic activation of endogenous c-Src, c-Yes, and Lyn, which are co-expressed in HeLa cells. c-Src, c-Yes, and Lyn were activated at different levels in M phase, and the activation was inhibited by Cdc2 inactivation. Mitotic c-Src and c-Yes exhibited normal- and retarded-electrophoretic-mobility forms on SDS-polyacrylamide gels, whereas Lyn did not show mobility retardation. Like c-Src, the retardation of electrophoretic mobility of c-Yes was caused by Cdc2-mediated phosphorylation. The normal- and retarded-mobility forms of c-Src were comparably activated, but activation of the retarded-mobility form of c-Yes was higher than that of the normal-mobility form of c-Yes. Thus, these results suggest that endogenous c-Src, c-Yes, and Lyn are differentially activated through Cdc2 activation during M phase.
AB - Src-family tyrosine kinases (SFKs) play an important role in mitosis. Despite overlapping expression of multiple SFK members, little is known about how individual SFK members are activated in M phase. Here, we examined mitotic activation of endogenous c-Src, c-Yes, and Lyn, which are co-expressed in HeLa cells. c-Src, c-Yes, and Lyn were activated at different levels in M phase, and the activation was inhibited by Cdc2 inactivation. Mitotic c-Src and c-Yes exhibited normal- and retarded-electrophoretic-mobility forms on SDS-polyacrylamide gels, whereas Lyn did not show mobility retardation. Like c-Src, the retardation of electrophoretic mobility of c-Yes was caused by Cdc2-mediated phosphorylation. The normal- and retarded-mobility forms of c-Src were comparably activated, but activation of the retarded-mobility form of c-Yes was higher than that of the normal-mobility form of c-Yes. Thus, these results suggest that endogenous c-Src, c-Yes, and Lyn are differentially activated through Cdc2 activation during M phase.
KW - Cdc2
KW - Electrophoretic mobility
KW - Fyn
KW - Lyn
KW - M phase
KW - Mitosis
KW - Phosphorylation
KW - Src-family tyrosine kinase
KW - c-Src
KW - c-Yes
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U2 - 10.1016/j.abb.2007.07.002
DO - 10.1016/j.abb.2007.07.002
M3 - Article
C2 - 17692281
AN - SCOPUS:34548625289
SN - 0003-9861
VL - 466
SP - 116
EP - 124
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 1
ER -