Differential induction of heat shock mRNA in oligodendrocytes, microglia, and astrocytes following hyperthermia

Jane A. Foster, Ian R. Brown

Research output: Contribution to journalArticlepeer-review

46 Scopus citations


A time course analysis of hsp70 mRNA induction in response to a physiologically relevant increase in body temperature of 2.6°C was performed in the rabbit forebrain. A protocol that combined in situ hybridization and cytochemistry on the same tissue section was employed to identify reactive glial cell types. Cytochemical markers for astrocytes, microglia, and oligodendrocytes were utilized in combination with a DIG-labelled hsp70 riboprobe, which permitted mRNA localization at high resolution. Four glial cell body-enriched regions of the rabbit forebrain were examined, namely, cortical layer 1, hippocampal fissure, corpus callosum, and fimbria. Maximal hsp70 mRNA induction was observed in 2 and 3 h hyperthermic animals. The colocalization analysis demonstrated that hsp70 mRNA was induced in oligodendrocytes and microglia, but not in forebrain GFAP positive astrocytes. In addition, cell counts were performed which showed that almost all oligodendrocytes induced hsp70 mRNA while a subpopulation of microglial cells responded. These data are consistent with the notion that oligodendrocytes, microglia, and astrocytes exhibit distinct thresholds for activation of the heat shock response following a physiologically relevant increase in body temperature.

Original languageEnglish (US)
Pages (from-to)207-218
Number of pages12
JournalMolecular Brain Research
Issue number2
StatePublished - May 1997
Externally publishedYes


  • Hsc70
  • Hsp70
  • Hybridization
  • In situ
  • Stress protein

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience


Dive into the research topics of 'Differential induction of heat shock mRNA in oligodendrocytes, microglia, and astrocytes following hyperthermia'. Together they form a unique fingerprint.

Cite this